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Relationships between transcription, silver staining and chromatin organization of nucleolar organizers in Secale cereale

2007Journal article Open access
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dc.contributor.authorCaperta, A.
dc.contributor.authorNeves, Nuno
dc.contributor.authorViegas, Wanda
dc.contributor.authorPikaard, Craig S.
dc.contributor.authorPreuss, Sasha
dc.date.accessioned2011-01-24T15:19:04Z
dc.date.available2011-01-24T15:19:04Z
dc.date.issued2007
dc.description.abstractSummary. The nucleolar organizer regions (NORs) are composed of hundreds of rRNA genes, typically spanning several megabases. Cytologically, NORs include regions that are highly condensed and regions that are decondensed, the latter corresponding to regions at which associated proteins stain intensively with silver (Ag-NORs) and where active rRNA gene transcription is thought to occur. To test the relationship between rRNA gene activity, NOR silver staining, and rDNA (genes coding for rRNA) chromatin condensation, we used the DNA methyltransferase inhibitor 5-azacytidine to evaluate the correlation between the epigenetic regulation of rRNA genes and NOR silver staining in the plant Secale cereale. Following 5-azacytidine treatment, we observed an increase in rRNA gene transcription as well as a reduction in the number of cells showing a significant difference in the size of the silver-stained domains in the two NORs. These transcriptional changes occurred concomitantly with an increase in nuclear and nucleolar size and were associated with the reallocation of most of the rDNA from perinucleolar heterochromatin into the nucleolus. Collectively, these results suggest that rRNA gene transcription, silver staining, and NOR decondensation are interrelated in S. cereale.por
dc.identifier.otherDOI 10.1007/s00709-007-0277-4
dc.identifier.urihttp://hdl.handle.net/10400.5/2847
dc.language.isoengpor
dc.publisherSpringerpor
dc.subjectnucleolar organizer regionpor
dc.subjecttranscriptionpor
dc.subjectsilver stainingpor
dc.subject5-Azacytidinepor
dc.subjectSecale cerealepor
dc.titleRelationships between transcription, silver staining and chromatin organization of nucleolar organizers in Secale cerealepor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.conferencePlaceAustriapor
oaire.citation.titleProtoplasmapor
rcaap.rightsopenAccesspor
rcaap.typearticlepor

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