Logo do repositório
 
Publicação

Negative synergistic impacts of ocean warming and acidification on the survival and proteome of the commercial sea bream, Sparus aurata

2018Artigo científico Acesso restrito
Ver registo simples
dc.contributor.authorAraújo, José E.
dc.contributor.authorMadeira, Diana
dc.contributor.authorVitorino, Rui
dc.contributor.authorRepolho, Tiago
dc.contributor.authorRosa, Rui
dc.contributor.authorDiniz, M.
dc.date.accessioned2020-01-19T20:28:06Z
dc.date.available2020-01-19T20:28:06Z
dc.date.issued2018
dc.description.abstractGlobal change is impacting aquatic ecosystems, with high risks for food production. However, the molecular underpinnings of organismal tolerance to both ocean warming and acidification are largely unknown. Here we tested the effect of warming and acidification in a 42-day experiment on a commercial temperate fish, the gilt-head seabream Sparus aurata. Juvenile fish were exposed to control (C 18 °C pH 8), ocean warming (OW 22 °C pH 8), ocean acidification (OA 18 °C pH 7.5) and ocean warming and acidification (OWA 22 °C pH 7.5). Proxies of fitness (mortality; condition index) and muscle proteome changes were assessed; bioinformatics tools (Cytoscape, STRAP, STRING) were used for functional analyses. While there was no mortality in fish under OW, fish exposed to OA and both OWA showed 17% and 50% mortality, respectively. Condition index remained constant in all treatments. OW alone induced small proteome adjustments (up-regulation of 2 proteins) related to epigenetic gene regulation and cytoskeletal remodeling. OA and both OWA induced greater proteome changes (12 and 8 regulated proteins, respectively) when compared to OW alone, suggesting that pH is central to proteome modulation. OA exposure led to increased glycogen degradation, glycolysis, lipid metabolism, anion homeostasis, cytoskeletal remodeling, immune processes and redox based signaling while decreasing ADP metabolic process. OWA led to increased lipid metabolism, glycogen degradation, glycolysis, cytoskeleton remodeling and decreased muscle filament sliding and intermediate filament organization. Moreover, as rates of change in temperature and acidification depend on region we tested as proof of concept an (i) acidification effect in a hot ocean (22 °C pH 8 vs 22 °C pH 7.5) which led to the regulation of 7 proteins, the novelty being in a boost of anaerobic metabolism and impairment of proteasomal degradation; and (ii) warming effect in an acidified ocean (18 °C pH 7.5 vs 22 °C pH 7.5) which led to the regulation of 5 proteins, with an emphasis on anaerobic metabolism and transcriptional regulation. The negative synergistic effects of ocean warming and acidification on fish survival coupled to the mobilization of storage compounds, enhancement in anaerobic pathways and impaired proteasomal degradation could pose a serious threat to the viability of sea bream populations.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.seares.2018.06.011pt_PT
dc.identifier.issn1385-1101
dc.identifier.urihttp://hdl.handle.net/10451/41175
dc.language.isoengpt_PT
dc.publisherElsevierpt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S1385110116303082pt_PT
dc.subjectGlobal changept_PT
dc.subjectProteomept_PT
dc.subjectPhenotypic plasticitypt_PT
dc.subjectFishpt_PT
dc.subjectTemperaturept_PT
dc.subjectpCO2pt_PT
dc.titleNegative synergistic impacts of ocean warming and acidification on the survival and proteome of the commercial sea bream, Sparus auratapt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage61pt_PT
oaire.citation.startPage50pt_PT
oaire.citation.titleJournal of Sea Researchpt_PT
oaire.citation.volume139pt_PT
person.familyNameMadeira
person.familyNameRepolho
person.familyNameRosa
person.givenNameDiana
person.givenNameTiago
person.givenNameRui
person.identifier440666
person.identifier430759
person.identifier.ciencia-id0313-5EB2-1A99
person.identifier.ciencia-id7812-9026-CAC2
person.identifier.ciencia-id2B10-7D61-FF7A
person.identifier.orcid0000-0002-2435-9283
person.identifier.orcid0000-0002-1048-8009
person.identifier.orcid0000-0003-2801-5178
person.identifier.ridN-3228-2013
person.identifier.ridA-4580-2009
person.identifier.scopus-author-id54401593300
person.identifier.scopus-author-id18435367300
person.identifier.scopus-author-id7102610088
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationbd766fe1-ae90-481e-9ce3-dae0f6e7b379
relation.isAuthorOfPublication0374386d-3719-4cf4-b44b-09c34fa94a1d
relation.isAuthorOfPublication395f77c0-ac42-4b7c-9b4a-07fdb75305cc
relation.isAuthorOfPublication.latestForDiscoverybd766fe1-ae90-481e-9ce3-dae0f6e7b379

Ficheiros

Principais
A mostrar 1 - 1 de 1
Miniatura indisponível
Nome:
MARE_ULisboa_JournalSeaResearch_139_2018_50-61.pdf
Tamanho:
1.95 MB
Formato:
Adobe Portable Document Format
Ver/Abrir
Licença
A mostrar 1 - 1 de 1
Miniatura indisponível
Nome:
license.txt
Tamanho:
1.2 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Ver/Abrir

Coleções

MARE - Artigos em Revistas Internacionais