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Photochemical features and trace element substituted chlorophylls as early detection biomarkers of metal exposure in the model diatom Phaeodactylum tricornutum

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dc.contributor.authorCabrita, Maria Teresa
dc.contributor.authorDuarte, Bernardo
dc.contributor.authorGameiro, C.
dc.contributor.authorGodinho, Rita M.
dc.contributor.authorCaçador, Isabel
dc.date.accessioned2019-06-25T09:47:15Z
dc.date.available2019-06-25T09:47:15Z
dc.date.issued2018
dc.description.abstractEstablishing marine species equipped with efficient biomarkers of trace element stress is a major requirement for the assessment of trace element contamination in marine ecosystems. This study investigates PS II overall activity and efficiency during light harvesting, electron transport chain (ETC) behaviour, OJIP-transient light curves, and pigment profiles, in the model marine diatom species Phaeodactylum tricornutum exposed to Cr, Co, Ni, Cu, Zn, Cd, Hg, Pb and to a mixture of all elements combined (Mix). OJIP-test parameters and trace element substituted chlorophylls were investigated as potential biomarkers of stress induced by trace elements. Hg-exposed cells showed a shift in balance towards PS I activity, promoted by increase in the electron transport from PQH2 to the reduction of PS I end electron acceptors. Other elements had more limited effects, mostly affecting energy dissipation and pigment levels to various degrees. Decoupling of PS II antennae connectivity was found in Co exposed cells. Chromium induced negative effects on PS I, but had no influence at the Oxygen Evolving Complexes (OECs) level. Copper caused a shift in the equilibrium between PS towards the PS I by reducing the PS II efficiency. Cadmium induced damage in OECs of the PS II donor side, but had no effect on the energy transduction pathway, maintaining energy processing efficiency. Lead had no significant effect on the energy transduction pathway, but produced changes at pigment level, leading to an increase in the chlorophyll and diadinoxanthin. In contrast, Ni had a beneficial role in P. tricornutum photochemistry, leading to increased number of available RC and oxidized quinone pool size, possibly because concentrations used were not able to induce severe cell damage. Copper, Cd and Zn led to substitution of Mg in chlorophyll a molecules, with the formation of less efficient CuChl a, CdChl a and ZnChl a. The biomarkers used evidenced the damaging effect of Co, Cu, Cr, Pb, Hg and all trace elements combined (Mix), pointing to Hg as the most harmful element. These physiological changes highlight P. tricornutum as a promising sentinel species for trace element contamination and the proposed photochemical features suitable as an efficient battery of biomarkers for trace element stress early detection in marine systems.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.ecolind.2017.07.057pt_PT
dc.identifier.issn1470-160X
dc.identifier.urihttp://hdl.handle.net/10451/38802
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.subjectOJIP-test parameterspt_PT
dc.subjectTrace element substituted chlorophyllspt_PT
dc.subjectBiomarkerspt_PT
dc.subjectPhaeodactylum tricornutumpt_PT
dc.subjectTrace element stress early detection and assessmentpt_PT
dc.subjectMarine coastal areaspt_PT
dc.titlePhotochemical features and trace element substituted chlorophylls as early detection biomarkers of metal exposure in the model diatom Phaeodactylum tricornutumpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage1052pt_PT
oaire.citation.startPage1038pt_PT
oaire.citation.titleEcological Indicatorspt_PT
oaire.citation.volume95pt_PT
person.familyNameDuarte
person.familyNameGameiro
person.familyNameCaçador
person.givenNameBernardo
person.givenNameCarla
person.givenNameIsabel
person.identifier89449
person.identifier.ciencia-id731E-093F-D4C8
person.identifier.ciencia-id771B-9D07-AB1A
person.identifier.ciencia-id631C-9FFE-CA81
person.identifier.orcid0000-0003-1914-7435
person.identifier.orcid0000-0003-2396-3929
person.identifier.orcid0000-0002-4475-6091
person.identifier.ridH-2001-2011
person.identifier.ridB-5462-2012
person.identifier.ridC-2618-2012
person.identifier.scopus-author-id20734149900
person.identifier.scopus-author-id20734253500
person.identifier.scopus-author-id6602533871
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationa72f0f40-c224-49ad-ba21-ebb5f9aab09c
relation.isAuthorOfPublication39490eaa-c76a-4478-9ff0-844df5fb91ca
relation.isAuthorOfPublication02ecb390-88b9-4e2f-8690-eba66c3b5311
relation.isAuthorOfPublication.latestForDiscovery39490eaa-c76a-4478-9ff0-844df5fb91ca

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