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Genomic and phenotypic characterization of Campylobacter fetus subsp. venerealis strains

2021-02-09Journal article Open access
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dc.contributor.authorSilva, Marta Filipa Serra da
dc.contributor.authorPereira, Ana L.
dc.contributor.authorFraqueza, M. J.
dc.contributor.authorPereira, Gonçalo
dc.contributor.authorMateus, Luisa
dc.contributor.authorLopes-da-Costa, Luís
dc.contributor.authorSilva, E
dc.date.accessioned2022-02-08T23:21:51Z
dc.date.available2022-02-08T23:21:51Z
dc.date.issued2021-02-09
dc.descriptionResearch Areas: Microbiologypt_PT
dc.description.abstractThe pathogenesis mechanisms of Campylobacter fetus subsp. venerealis (Cfv), the etiologic agent of Bovine Genital Campylobacteriosis remain elusive. This study evaluated the virulence potential and biovar characteristics of Cfv isolates (n = 13) by PCR screening of putative virulencefactor (VF) genes, Multilocus Sequence Typing (MLST) analysis, antimicrobial susceptibility to tetracycline, penicillin, enrofloxacin and streptomycin testing and whole-genome sequencing (WGS; n = 5), also comparing the latter with 26 other whole-genome sequences of Cfv strains. The putative VF genes encoding type IV secretion system of Cfv (virB2-virB11/virD4) were absent in 92% of isolates, including isolates from aborted foetuses, evidencing that these VF genes are not essential for Cfv pathogenicity. The parA gene, used as a Cfv diagnostic molecular target, was detected in only 3 of 13 isolates, invalidating its use for diagnosis purposes. Three novel sequence types were identified by MLST. Although no in vitro antimicrobial resistance was detected, WGS identified antimicrobial resistance-related genes, including those encoding the multidrug efflux pumps CmeABC and YkkCD, indicating that their presence is not enough to provide antimicrobial resistance. The SNP and accessory protein families analysis segregated the Cfv and Cfv biovar intermedius (Cfvi) strains into different clusters. In conclusion, this study evidenced virulence potential and biovar characteristics of Cfv and Cfvi, which are of relevance for the control of Bovine Genital Campylobacteriosis.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSilva MF, Pereira AL, Fraqueza MJ, Pereira G, Mateus L, Lopes-da-Costa L, Silva E. 2021. Genomic and phenotypic characterization of Campylobacter fetus subsp. venerealis strains. Microorganisms, 9(2):340. Doi: 10.3390/microorganisms9020340pt_PT
dc.identifier.doi10.3390/microorganisms9020340pt_PT
dc.identifier.eissn2076-2607
dc.identifier.urihttp://hdl.handle.net/10400.5/23440
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationUIDP/CVT/00276/2020pt_PT
dc.relationBiotechnological Innovative Solutions for the Control of Bovine Genital Campylobacteriosis
dc.relationCampylobacter-host interaction in bovine genital campilobacteriosis
dc.relationalterado para: “Cytological endometritis in dairy cattle: news insights into pathogenesis, diagnosis and therapy”. Effects of Bovine Venereal Campylobacteriosis and Bovine Viral Diarrohea interaction on beef herd reproductive efficiency
dc.relation.publisherversionhttps://www.mdpi.com/2076-2607/9/2/340pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectCampylobacter fetus subsp. venerealispt_PT
dc.subjectBiovar intermediuspt_PT
dc.subjectMultilocus sequencing typingpt_PT
dc.subjectVirulence potentialpt_PT
dc.subjectAntimicrobial susceptibilitypt_PT
dc.subjectGenomic characterizationpt_PT
dc.titleGenomic and phenotypic characterization of Campylobacter fetus subsp. venerealis strainspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleBiotechnological Innovative Solutions for the Control of Bovine Genital Campylobacteriosis
oaire.awardTitleCampylobacter-host interaction in bovine genital campilobacteriosis
oaire.awardTitlealterado para: “Cytological endometritis in dairy cattle: news insights into pathogenesis, diagnosis and therapy”. Effects of Bovine Venereal Campylobacteriosis and Bovine Viral Diarrohea interaction on beef herd reproductive efficiency
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC%2FCVT-CVT%2F30145%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F125657%2F2016/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F130923%2F2017/PT
oaire.citation.conferencePlaceBasel, Switzerlandpt_PT
oaire.citation.titleMicroorganismspt_PT
oaire.citation.volumeVol. 9(2):340pt_PT
oaire.fundingStream9471 - RIDTI
person.familyNameSerra da Silva
person.familyNamePereira
person.familyNameFraqueza
person.familyNameMaria Freire Leal Mateus
person.familyNameLopes-da-Costa
person.familyNameTomé Sousa Silva
person.givenNameMarta Filipa
person.givenNameAna Lúcia
person.givenNameMaria João
person.givenNameLuisa
person.givenNameLuís
person.givenNameMaria Elisabete
person.identifierM-8503-2013
person.identifier.ciencia-id511A-5A1A-AEDC
person.identifier.ciencia-idCA18-CCF2-652B
person.identifier.ciencia-id101C-DBCC-1D3F
person.identifier.ciencia-id0A1F-AC68-0919
person.identifier.ciencia-id651A-9A4A-1CE0
person.identifier.orcid0000-0001-9479-5550
person.identifier.orcid0000-0002-8388-0511
person.identifier.orcid0000-0002-8304-9920
person.identifier.orcid0000-0002-2332-7642
person.identifier.ridK-3819-2013
person.identifier.scopus-author-id14829172700
person.identifier.scopus-author-id22953692100
person.identifier.scopus-author-id6701529486
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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