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An update on wastewater multi-resistant bacteria : identification of clinical pathogens such as Escherichia coli O25b:H4-B2-ST131-producing CTX-M-15 ESBL and KPC-3 Carbapenemase-Producing Klebsiella oxytoca

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dc.contributor.authorMesquita, Elsa
dc.contributor.authorRibeiro, Rita
dc.contributor.authorSilva, Carla J. C.
dc.contributor.authorAlves, Rita
dc.contributor.authorBaptista, Rita
dc.contributor.authorCondinho, Sílvia
dc.contributor.authorRosa, Maria João
dc.contributor.authorPerdigão, João
dc.contributor.authorCaneiras, Catia
dc.contributor.authorDuarte, Aida
dc.date.accessioned2021-04-21T12:01:05Z
dc.date.available2021-04-21T12:01:05Z
dc.date.issued2021
dc.description© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).pt_PT
dc.description.abstractWastewater treatment plants (WWTPs) are significant reservoirs of bacterial resistance. This work aims to identify the determinants of resistance produced by Gram-negative bacteria in the influent and effluent of two WWTPs in Portugal. A total of 96 wastewater samples were obtained between 2016 and 2019. The numbers of total aerobic and fecal contamination bacteria were evaluated, and genomic features were searched by polymerase chain reaction (PCR) and Next-Generation Sequencing (NGS). Enterobacteriaceae corresponded to 78.6% (n = 161) of the 205 isolates identified by 16sRNA. The most frequent isolates were Escherichia spp. (57.1%, n = 117), followed by Aeromonas spp. (16.1%, n = 33) and Klebsiella spp. (12.7%, n = 26). The remaining 29 isolates (14.1%) were distributed across 10 different genera. Among the 183 resistant genes detected, 54 isolates produced extended spectrum β-lactamases (ESBL), of which blaCTX-M-15 was predominant (37 isolates; 68.5%). A KPC-3 carbapenemase-producing K. oxytoca was identified (n = 1), with blaKPC-3 included in a transposon Tn4401 isoform b. A higher number of virulence genes (VG) (19 genes) was found in the E. coli 5301 (O25b-ST131-B2) isolate compared with a commensal E. coli 5281 (O25b-ST410-A) (six genes). Both shared five VG [Enterobactin; Aerobactin, CFA/1 (clade α); Type1 (clade γ1); Type IV]. In conclusion, this work highlights the role of relevant clinical bacteria in WWTPs, such as KPC-3-producing K. oxytoca, and, for the first time, a CTX-M-15-producing Ochromobactrum intermedium, a human opportunistic pathogen, and a SED-1-producing Citrobacter farmeri, an uncommon CTX-M-type extended-spectrum beta-lactamase.pt_PT
dc.description.sponsorshipThis work was supported by the European Union LIFE Programme under Grant Agreement LIFE14 ENV/PT/000739—LIFE Impetus (https://life-impetus.eu/), accessed on 1 February 2021.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMicroorganisms. 2021 Mar 11;9(3):576pt_PT
dc.identifier.doi10.3390/microorganisms9030576pt_PT
dc.identifier.eissn2076-2607
dc.identifier.urihttp://hdl.handle.net/10451/47498
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationLIFE14 ENV/PT/000739pt_PT
dc.relation.publisherversionhttps://www.mdpi.com/journal/microorganismspt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectWastewater treatment plant (WWTP)pt_PT
dc.subjectCTX-M-15pt_PT
dc.subjectSED-1pt_PT
dc.subjectESBLpt_PT
dc.subjectKPC-3 carbapenemasept_PT
dc.subjectKlebsiella oxytocapt_PT
dc.subjectOchromobactrum intermediumpt_PT
dc.subjectCitrobacter farmeript_PT
dc.subjectPortugalpt_PT
dc.titleAn update on wastewater multi-resistant bacteria : identification of clinical pathogens such as Escherichia coli O25b:H4-B2-ST131-producing CTX-M-15 ESBL and KPC-3 Carbapenemase-Producing Klebsiella oxytocapt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue3pt_PT
oaire.citation.titleMicroorganismspt_PT
oaire.citation.volume9pt_PT
person.familyNameLucas Mota Perdigão
person.familyNameCaneiras
person.familyNameDuarte
person.givenNameJoão Ruben
person.givenNameCatia
person.givenNameAida
person.identifier2524696
person.identifier.ciencia-id991C-F26A-9843
person.identifier.ciencia-id5217-449F-3B4F
person.identifier.ciencia-idD517-F4DB-17C1
person.identifier.orcid0000-0002-0339-1305
person.identifier.orcid0000-0002-3735-8554
person.identifier.orcid0000-0002-0616-4650
person.identifier.ridM-5272-2013
person.identifier.scopus-author-id24723211800
person.identifier.scopus-author-id35495748300
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication26de40fc-fc1d-4ff9-a242-3bb398e3ac1e
relation.isAuthorOfPublication67e72892-1980-4138-aeeb-244c4222d335
relation.isAuthorOfPublicationf7620f51-a5ef-4e71-9cf5-3f34eb294a9d
relation.isAuthorOfPublication.latestForDiscovery67e72892-1980-4138-aeeb-244c4222d335

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