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Effect of α-tocopherol on the physicochemical, antioxidant and antibacterial properties of levofloxacin loaded hybrid lipid nanocarriers

2021Artigo científico Acesso aberto
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dc.contributor.authorIslan, Germán A.
dc.contributor.authorGonçalves, Lídia
dc.contributor.authorMarto, Joana
dc.contributor.authorDuarte, Aida
dc.contributor.authorAlvarez, Vera A.
dc.contributor.authorCastro, Guillermo R.
dc.contributor.authorAlmeida, António José
dc.date.accessioned2025-08-14T20:00:06Z
dc.date.available2025-08-14T20:00:06Z
dc.date.issued2021
dc.date.updated2023-02-27T16:12:50Z
dc.description.abstractIn the global context of emerging pathogens associated with respiratory diseases, novel nanostructured lipid carriers (NLCs) having both antioxidant and antimicrobial properties produced by α-tocopherol (αT) and levofloxacin (LV), respectively, were developed. The hybrid NLCs were made of solid lipid esters and liquid αT as the central core, plus chitosan (Chi) and poloxamer 188 as surface stabilizers. Three formulations were rationally designed considering lipids with different physicochemical properties: cetyl ester (SS), glyceryl dibehenate (CO) and glyceryl tristearate (DY). The properties and stability of the nanoformulations were studied by dynamic light scattering considering the effects of the homogenization time, surfactant concentration, and initial lipid amount on the mean size, polydispersity index (PDI) and zeta potential of the nanoparticles. The effect of αT on the nanostructuration of the lipid matrix was demonstrated by X-ray diffraction, differential scanning calorimetry, and thermogravimetry analysis. The potential ability of the NLCs to interact with the mucosa was studied by rheological methods, demonstrating a reduction in mucin cohesiveness and elasticity due to the mucoadhesive properties of NLCs coated with chitosan. Solid lipid nanoparticles (SLNs) of SS, CO and DY encapsulated 58.0 ± 3.2, 25.7 ± 7.1, and 27.9 ± 1.5% LV, respectively, while the NLC formulations were able to encapsulate 80–90% LV and allowed controlled release of the drug. The enhanced antimicrobial properties of the SLNs/NLCs against Pseudomonas aeruginosa and Staphylococcus aureus were confirmed. The presence of αT in the formulations provided the antioxidant properties of the NLCs. Finally, no acute toxicities of the formulations were observed in two human lung cell lines (A549 and Calu-3).pt_PT
dc.description.sponsorshipThe present work was supported by Argentine grants from CONICET (National Council for Science and Technology, PIP0498), The National Agency of Scientific and Technological Promotion (ANPCyT, PICT2016-4597, PICT2016-4597), UNLP (Nat. Univ. of La Plata, X815) and Fundación Argentina de Nanotecnología (FAN) to GRC. Also, GAI wants to thank the Grants “Subsidio para Jóvenes Investigadores UNLP 2017” and “PICT Jóvenes 2017-2251” for financing some reagents. The authors are also grateful to Fundação para a Ciência e a Tecnologia (FCT), Portugal, for the financial support under iMED.ULisboa project Pest-UID/DTP/04138/2019. Finally, we want to thank CRODA Argentina for kindly donating the lipids, and MSc. A. Torres Nicolini, Ms M. Bracone, and Ms Ignacio Rivero Berti for their collaboration in physicochemical tests.pt_PT
dc.description.versioninfo:eu-repo/semantics/acceptedVersionpt_PT
dc.identifier.citationIslan GA, Gonçalves LMD, Marto J, Duarte A, Alvarez VA, Castro GR, et al. Effect of α-tocopherol on the physicochemical, antioxidant and antibacterial properties of levofloxacin loaded hybrid lipid nanocarriers. New J Chem 2021;45:1029–42. https://doi.org/10.1039/D0NJ03781H.pt_PT
dc.identifier.doi10.1039/d0nj03781hpt_PT
dc.identifier.issn1144-0546
dc.identifier.issn1369-9261
dc.identifier.slugcv-prod-2118907
dc.identifier.urihttp://hdl.handle.net/10400.5/102923
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherRoyal Society of Chemistrypt_PT
dc.relationResearch Institute for Medicines
dc.relation.publisherversionhttps://pubs.rsc.org/en/content/articlelanding/2021/nj/d0nj03781hpt_PT
dc.titleEffect of α-tocopherol on the physicochemical, antioxidant and antibacterial properties of levofloxacin loaded hybrid lipid nanocarrierspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleResearch Institute for Medicines
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FDTP%2F04138%2F2019/PT
oaire.citation.endPage1042pt_PT
oaire.citation.issue2pt_PT
oaire.citation.startPage1029pt_PT
oaire.citation.titleNew Journal of Chemistrypt_PT
oaire.citation.volume45pt_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameDiogo Gonçalves
person.familyNameMarques Marto
person.familyNameDuarte
person.familyNameLeitão das Neves Almeida
person.givenNameLídia Maria
person.givenNameJoana
person.givenNameAida
person.givenNameAntónio José
person.identifier2524696
person.identifierI-6590-2012
person.identifier.ciencia-id7211-22BA-86AD
person.identifier.ciencia-id891B-12E1-C2BF
person.identifier.ciencia-idD517-F4DB-17C1
person.identifier.ciencia-id8312-F853-C47A
person.identifier.orcid0000-0002-6799-2740
person.identifier.orcid0000-0001-5523-5622
person.identifier.orcid0000-0002-0616-4650
person.identifier.orcid0000-0002-7807-4726
person.identifier.ridM-5272-2013
person.identifier.scopus-author-id35495748300
person.identifier.scopus-author-id57195052432
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.cv.cienciaid7211-22BA-86AD | Lídia Maria Diogo Gonçalves
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication3265148d-dc2d-4601-a832-2e72f7be5662
relation.isAuthorOfPublication07221f19-3d9a-4bd5-88c4-1e066b49856f
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