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Comparative analysis of the bronchoalveolar microbiome in Portuguese patients with different chronic lung disorders

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dc.contributor.authorSeixas, Susana
dc.contributor.authorKolbe, Allison R.
dc.contributor.authorGomes, Sílvia
dc.contributor.authorSucena, Maria
dc.contributor.authorSousa, Catarina
dc.contributor.authorVaz Rodrigues, Luís
dc.contributor.authorTeixeira, Gilberto
dc.contributor.authorPinto, Paula
dc.contributor.authorTavares de Abreu, Tiago
dc.contributor.authorBárbara, Cristina
dc.contributor.authorSemedo, Júlio
dc.contributor.authorMota, Leonor
dc.contributor.authorCarvalho, Ana Sofia
dc.contributor.authorMatthiesen, Rune
dc.contributor.authorMarques, Patrícia Isabel
dc.contributor.authorPérez-Losada, Marcos
dc.date.accessioned2021-07-26T12:43:52Z
dc.date.available2021-07-26T12:43:52Z
dc.date.issued2021
dc.description© The Author(s) 2021. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.pt_PT
dc.description.abstractThe lung is inhabited by a diverse microbiome that originates from the oropharynx by a mechanism of micro-aspiration. Its bacterial biomass is usually low; however, this condition shifts in lung cancer (LC), chronic obstructive pulmonary disease (COPD) and interstitial lung disease (ILD). These chronic lung disorders (CLD) may coexist in the same patient as comorbidities and share common risk factors, among which the microbiome is included. We characterized the microbiome of 106 bronchoalveolar lavages. Samples were initially subdivided into cancer and non-cancer and high-throughput sequenced for the 16S rRNA gene. Additionally, we used a cohort of 25 CLD patients where crossed comorbidities were excluded. Firmicutes, Proteobacteria and Bacteroidetes were the most prevalent phyla independently of the analyzed group. Streptococcus and Prevotella were associated with LC and Haemophilus was enhanced in COPD versus ILD. Although no significant discrepancies in microbial diversity were observed between cancer and non-cancer samples, statistical tests suggested a gradient across CLD where COPD and ILD displayed the highest and lowest alpha diversities, respectively. Moreover, COPD and ILD were separated in two clusters by the unweighted UniFrac distance (P value = 0.0068). Our results support the association of Streptoccocus and Prevotella with LC and of Haemophilus with COPD, and advocate for specific CLD signatures.pt_PT
dc.description.sponsorshipThis work was supported by Norte Portugal Regional Programme (NORTE 2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (FEDER)—project NORTE-01-0145-FEDER-000029. This work was also financed by FEDER funds through COMPETE 2020 (Operacional Programme for Competitiveness and Internationalization— POCI) and by private funds through the Young Investigator Prize Francisco Augusto da Fonseca Dias and Maria José Melenas da Fonseca Dias to P.I.M. FCT supports P.I.M. through a post-doctoral fellowship (SFRH/BPD/120777/2016), financed by the Portuguese State Budget of the Ministry for Science, Technology and High Education and from the European Social Fund (Programa Operacional do Capital Humano—POCH). M.P-L was partially supported by FCT under the Programa Operacional Potencial Humano—Quadro de Referência Estratégico Nacional funds from the European Social Fund and Portuguese State Budget of the Ministry for Science, Technology and High Education (IF/00764/2013; RX: IF/00359/2015).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSci Rep. 2021 Jul 22;11(1):15042pt_PT
dc.identifier.doi10.1038/s41598-021-94468-ypt_PT
dc.identifier.eissn2045-2322
dc.identifier.urihttp://hdl.handle.net/10451/49111
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringer Naturept_PT
dc.relationNORTE-01-0145-FEDER-000029pt_PT
dc.relationUnderstanding the basis of semen hyperviscosity and asthenozoospermia phenotypes - a systems biology approach
dc.relationTo be or not to be diseased: the effects of microbiome on susceptibility and resistance to parasitic diseases in farmed seabream and seabass
dc.relation.publisherversionhttps://www.nature.com/srep/pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.titleComparative analysis of the bronchoalveolar microbiome in Portuguese patients with different chronic lung disorderspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardNumberSFRH/BPD/120777/2016
oaire.awardNumberIF/00764/2013/CP1154/CT0010
oaire.awardNumberIF/00359/2015/CP1278/CT0003
oaire.awardTitleUnderstanding the basis of semen hyperviscosity and asthenozoospermia phenotypes - a systems biology approach
oaire.awardTitleTo be or not to be diseased: the effects of microbiome on susceptibility and resistance to parasitic diseases in farmed seabream and seabass
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F120777%2F2016/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/Investigador FCT/IF%2F00764%2F2013%2FCP1154%2FCT0010/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/Investigador FCT/IF%2F00359%2F2015%2FCP1278%2FCT0003/PT
oaire.citation.issue1pt_PT
oaire.citation.titleScientific Reportspt_PT
oaire.citation.volume11pt_PT
oaire.fundingStreamInvestigador FCT
oaire.fundingStreamInvestigador FCT
person.familyNamePinto
person.familyNameBárbara
person.givenNamePaula Maria Gonçalves
person.givenNameCristina
person.identifier1330713
person.identifier.ciencia-idF01D-6AFB-DFFD
person.identifier.ciencia-id171F-DE21-B23B
person.identifier.orcid0000-0003-2301-2752
person.identifier.orcid0000-0003-0915-4105
person.identifier.scopus-author-id24074744900
person.identifier.scopus-author-id6701574502
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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