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Nonlinear emission of quinolizinium-based dyes with application in fluorescence lifetime imaging

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dc.contributor.authorMarcelo, Gema
dc.contributor.authorPinto, Sandra
dc.contributor.authorCañeque, Tatiana
dc.contributor.authorMariz, Inês F. A.
dc.contributor.authorVaquero, Juan J.
dc.contributor.authorMartinho, J. M. G.
dc.contributor.authorMaçôas, Ermelinda
dc.contributor.authorCuadro, Ana M.
dc.date.accessioned2022-05-26T16:43:19Z
dc.date.available2022-05-26T16:43:19Z
dc.date.issued2014
dc.description© 2014 American Chemical Society.pt_PT
dc.description.abstractCharged molecules based on the quinolizinum cation have potential applications as labels in fluorescence imaging in biological media under nonlinear excitation. A systematic study of the linear and nonlinear photophysics of derivatives of the quinolizinum cation substituted by either dimethylaniline or methoxyphenyl electron donors is performed. The effects of donor strength, conjugation length, and symmetry in the two-photon emission efficiency are analyzed in detail. The best performing nonlinear fluorophore, with two-photon absorption cross sections of 1140 GM and an emission quantum yield of 0.22, is characterized by a symmetric D-π-A(+)-π-D architecture based on the methoxyphenyl substituent. Application of this molecule as a fluorescent marker in optical microscopy of living cells revealed that, under favorable conditions, the fluorophore can be localized in the cytoplasmatic compartment of the cell, staining vesicular shape organelles. At higher dye concentrations and longer staining times, the fluorophore can also penetrate into the nucleus. The nonlinearly excited fluorescence lifetime imaging shows that the fluorophore lifetime is sensitive to its location in the different cell compartments. Using fluorescence lifetime microscopy, a multicolor map of the cell is drafted with a single dye.pt_PT
dc.description.sponsorshipThe authors acknowledge financial support from the Spanish Ministerio de Economía y Competitividad (Project CTQ2011-24715), the Portuguese Fundação para a Ciência e a Tecnologia (Projects PTDC/CTM-POL/114367/2009, RECI/CTM-POL/0342/2012) and a grant from the Universidad de Alcalá (TC).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationJ Phys Chem A. 2015 Mar 19;119(11):2351-2362pt_PT
dc.identifier.doi10.1021/jp507095bpt_PT
dc.identifier.eissn1520-5215
dc.identifier.issn1089-5639
dc.identifier.urihttp://hdl.handle.net/10451/53198
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherACS Publicationspt_PT
dc.relation.publisherversionhttps://pubs.acs.org/journal/jpcafhpt_PT
dc.titleNonlinear emission of quinolizinium-based dyes with application in fluorescence lifetime imagingpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FCTM-POL%2F114367%2F2009/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/RECI%2FCTM-POL%2F0342%2F2012/PT
oaire.citation.endPage2362pt_PT
oaire.citation.issue11pt_PT
oaire.citation.startPage2351pt_PT
oaire.citation.titleThe Journal of Physical Chemistry Apt_PT
oaire.citation.volume119pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream3599-PPCDT
person.familyNamemarcelo
person.familyNamePinto
person.familyNameAfonso Mariz
person.familyNameMartinho
person.familyNameMaçôas
person.givenNamegema
person.givenNameSandra
person.givenNameInês de Fátima
person.givenNameJose
person.givenNameErmelinda
person.identifier996963
person.identifier.ciencia-idDF1B-B557-85AF
person.identifier.ciencia-id901E-983E-0177
person.identifier.ciencia-id9816-C90F-56BC
person.identifier.ciencia-idA21C-8770-B06E
person.identifier.orcid0000-0003-4955-7400
person.identifier.orcid0000-0002-3205-9459
person.identifier.orcid0000-0001-5699-724X
person.identifier.orcid0000-0003-1806-1568
person.identifier.orcid0000-0001-8506-7025
person.identifier.ridB-4378-2009
person.identifier.ridH-8446-2016
person.identifier.ridN-6691-2015
person.identifier.ridE-1988-2013
person.identifier.ridB-2989-2010
person.identifier.scopus-author-id6508249653
person.identifier.scopus-author-id25637336300
person.identifier.scopus-author-id55325394100
person.identifier.scopus-author-id7005987755
person.identifier.scopus-author-id6602705500
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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