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Involvement of disperse repetitive sequences in wheat/rye genome adjustment
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Orientador(es)
Resumo(s)
The union of different genomes in the same nucleus frequently results in hybrid
genotypes with improved genome plasticity related to both genome remodeling events and
changes in gene expression. Most modern cereal crops are polyploid species. Triticale,
synthesized by the cross between wheat and rye, constitutes an excellent model to study
polyploidization functional implications. We intend to attain a deeper knowledge of
dispersed repetitive sequence involvement in parental genome reshuffle in triticale and in
wheat-rye addition lines that have the entire wheat genome plus each rye chromosome pair.
Through Random Amplified Polymorphic DNA (RAPD) analysis with OPH20 10-mer
primer we unraveled clear alterations corresponding to the loss of specific bands from both
parental genomes. Moreover, the sequential nature of those events was revealed by the
increased absence of rye-origin bands in wheat-rye addition lines in comparison with
triticale. Remodeled band sequencing revealed that both repetitive and coding genome
domains are affected in wheat-rye hybrid genotypes. Additionally, the amplification and
sequencing of pSc20H internal segments showed that the disappearance of parental bands
may result from restricted sequence alterations and unraveled the involvement of wheat/rye
related repetitive sequences in genome adjustment needed for hybrid plant stabilization
Descrição
Palavras-chave
genome adjustment triticale wheat-rye addition lines dispersed repetitive sequences pSc20H
Contexto Educativo
Citação
"International Journal of Molecular Sciences". ISSN 1442-0067. 13 (2012). 8549-8561