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Excreted Trypanosoma brucei proteins inhibit Plasmodium hepatic infection

2021Journal article Open access
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dc.contributor.authorTemporão, Adriana
dc.contributor.authorSanches-Vaz, Margarida
dc.contributor.authorLuís, Rafael
dc.contributor.authorNunes-Cabaço, Helena
dc.contributor.authorSmith, Terry K.
dc.contributor.authorPrudêncio, Miguel
dc.contributor.authorFigueiredo, Luisa M.
dc.date.accessioned2023-02-23T15:19:20Z
dc.date.available2023-02-23T15:19:20Z
dc.date.issued2021
dc.description© 2021 Temporão et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.pt_PT
dc.description.abstractMalaria, a disease caused by Plasmodium parasites, remains a major threat to public health globally. It is the most common disease in patients with sleeping sickness, another parasitic illness, caused by Trypanosoma brucei. We have previously shown that a T. brucei infection impairs a secondary P. berghei liver infection and decreases malaria severity in mice. However, whether this effect requires an active trypanosome infection remained unknown. Here, we show that Plasmodium liver infection can also be inhibited by the serum of a mouse previously infected by T. brucei and by total protein lysates of this kinetoplastid. Biochemical characterisation showed that the anti-Plasmodium activity of the total T. brucei lysates depends on its protein fraction, but is independent of the abundant variant surface glycoprotein. Finally, we found that the protein(s) responsible for the inhibition of Plasmodium infection is/are present within a fraction of ~350 proteins that are excreted to the bloodstream of the host. We conclude that the defence mechanism developed by trypanosomes against Plasmodium relies on protein excretion. This study opens the door to the identification of novel antiplasmodial intervention strategies.pt_PT
dc.description.sponsorshipThis work was supported by the Howard Hughes Medical Institute (ref. 55007419) awarded to LMF, by Fundação para a Ciência e Tecnologia (PD/BD/138891/2018) awarded to AT, (PD/BD/105838/2018) awarded to MSV, (CEECIND/03322/2018) awarded to LMF, (CEECIND/03539/2017) awarded to MP, and by the Wellcome Trust (094476/Z/10/Z) awarded to TKS.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationPLoS Negl Trop Dis . 2021 Oct 29;15(10):e0009912pt_PT
dc.identifier.doi10.1371/journal.pntd.0009912pt_PT
dc.identifier.eissn1935-2735
dc.identifier.issn1935-2727
dc.identifier.urihttp://hdl.handle.net/10451/56428
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherPLOSpt_PT
dc.relationPD/BD/105838/2018pt_PT
dc.relationUnraveling the role of Trypanosoma brucei proteins in the impairment of Plasmodium infection
dc.relationNot Available
dc.relationNot Available
dc.relation.publisherversionhttps://journals.plos.org/plosntds/pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.titleExcreted Trypanosoma brucei proteins inhibit Plasmodium hepatic infectionpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleUnraveling the role of Trypanosoma brucei proteins in the impairment of Plasmodium infection
oaire.awardTitleNot Available
oaire.awardTitleNot Available
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//PD%2FBD%2F138891%2F2018/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/CEEC IND 2018/CEECIND%2F03322%2F2018%2FCP1543%2FCT0009/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/CEEC IND 2017/CEECIND%2F03539%2F2017%2FCP1396%2FCT0009/PT
oaire.citation.issue10pt_PT
oaire.citation.titlePLOS Neglected Tropical Diseasespt_PT
oaire.citation.volume15pt_PT
oaire.fundingStreamCEEC IND 2018
oaire.fundingStreamCEEC IND 2017
person.familyNameTemporão
person.familyNameSanches-Vaz
person.familyNameLuís
person.familyNameNunes Cabaço
person.familyNamePrudêncio
person.familyNameFigueiredo
person.givenNameAdriana
person.givenNameMargarida
person.givenNameRafael
person.givenNameHelena Isabel Marques
person.givenNameMiguel
person.givenNameLuisa M
person.identifierhttps://scholar.google.pt/citations?user=zduN6wsAAAAJ&hl=pt-PT&oi=ao
person.identifier.ciencia-id8E1C-2069-D56C
person.identifier.ciencia-idD01D-34B6-6C79
person.identifier.ciencia-id5511-16ED-48E0
person.identifier.ciencia-id421C-420D-E7BD
person.identifier.orcid0000-0002-1208-9984
person.identifier.orcid0000-0002-0741-6127
person.identifier.orcid0000-0002-8305-1625
person.identifier.orcid0000-0001-7213-2879
person.identifier.orcid0000-0003-1746-6029
person.identifier.orcid0000-0002-5752-6586
person.identifier.scopus-author-id6603561872
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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