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An in vitro study of adrenaline effect on human erythrocyte properties in both gender
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Orientador(es)
Resumo(s)
The possibility that erythrocytes may function as a reservoir for noradrenaline and adrenaline and as a modulator
of circulating catecholamine concentrations had been suggested. The aim of this work was to study the adrenaline effect on erythrocyte membrane fluidity, acetylcholinesterase (AChE) enzyme activity, P50 and erythrocyte deformability and also to
verify if the role of adrenaline on erythrocyte properties is sex-dependent. Blood samples from 42 healthy donors were obtained, and its aliquots incubated 30 min without (control) and with 10−5 M concentrations of adrenaline alone (A1) and adrenaline with an α and an β-blocker (A2). Results demonstrate that initial AChE values in female are higher (p≤0.01) than male values.
In female, adrenaline decreases AChE activity either when α and β-adrenergic receptors are blocked (p≤0.01) or when they
are not. In male, adrenaline increases AChE activity when none of adrenergic receptors are blocked. Control values of male
and female erythrocyte membrane fluidity are very similar but behaviour became differently (p≤0.05) when adrenaline is
present because it decreases male and increases female values. Gender differences in erythrocyte deformability are verified
at high shear stress values (p≤0.02). In female we have also registered the existence of an inverse significant correlation
(r = −0.62) between membrane rigidity and AChE activity in A2 values. Adrenaline increases p50 values (p≤0.03) in both
sexes. Peripheral blood film has shown echinocytes when adrenaline 10−5 M is present. We conclude that in this in vitro study sex-related differences in erythrocyte acetylcholinesterase enzyme activity, membrane fluidity and erythrocyte deformability under adrenaline influence were found.
Descrição
© 2003 – IOS Press. All rights reserved
Palavras-chave
Adrenaline Erythrocyte membrane fluidity Acetylcholinesterase enzyme activity P50 Erythrocyte Gender
Contexto Educativo
Citação
Clinical Hemorheology and Microcirculation 28 (2003) 89–98