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Age-associated distribution of normal B-cell and plasma cell subsets in peripheral blood

2018Journal article Open access
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dc.contributor.authorBlanco, Elena
dc.contributor.authorPérez-Andrés, Martín
dc.contributor.authorArriba-Méndez, Sonia
dc.contributor.authorContreras-Sanfeliciano, Teresa
dc.contributor.authorCriado, Ignacio
dc.contributor.authorPelak, Ondrej
dc.contributor.authorSerra-Caetano, Ana
dc.contributor.authorRomero, Alfonso
dc.contributor.authorPuig, Noemí
dc.contributor.authorRemesal, Ana
dc.contributor.authorTorres Canizales, Juan
dc.contributor.authorLópez-Granados, Eduardo
dc.contributor.authorKalina, Tomas
dc.contributor.authorSousa, Ana E.
dc.contributor.authorvan Zelm, Menno
dc.contributor.authorvan der Burg, Mirjam
dc.contributor.authorvan Dongen, Jacques J. M.
dc.contributor.authorOrfao, Alberto
dc.date.accessioned2022-05-19T13:27:52Z
dc.date.available2022-05-19T13:27:52Z
dc.date.issued2018
dc.description© 2018 The Authors. Published by Elsevier Inc. on behalf of the American Academy of Allergy, Asthma & Immunology. This is an open access article under the CC BY-NC- ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).pt_PT
dc.description.abstractBackground: Humoral immunocompetence develops stepwise throughout life and contributes to individual susceptibility to infection, immunodeficiency, autoimmunity, and neoplasia. Immunoglobulin heavy chain (IgH) isotype serum levels can partly explain such age-related differences, but their relationship with the IgH isotype distribution within memory B-cell (MBC) and plasma cell (PCs) compartments remains to be investigated. Objective: We studied the age-related distribution of MBCs and PCs expressing different IgH isotypes in addition to the immature/transitional and naive B-cell compartments. Methods: B-cell and PC subsets and plasma IgH isotype levels were studied in cord blood (n = 19) and peripheral blood (n = 215) from healthy donors aged 0 to 90 years by using flow cytometry and nephelometry, respectively. Results: IgH-switched MBCs expressing IgG1, IgG2, IgG3, IgA1, and IgA2 were already detected in cord blood and newborns at very low counts, whereas CD27+IgM++IgD+ MBCs only became detectable at 1 to 5 months and remained stable until 2 to 4 years, and IgD MBCs peaked at 2 to 4 years, with both populations decreasing thereafter. MBCs expressing IgH isotypes of the second immunoglobulin heavy chain constant region (IGHC) gene block (IgG1, IgG3, and IgA1) peaked later during childhood (2-4 years), whereas MBCs expressing third IGHC gene block immunoglobulin isotypes (IgG2, IgG4, and IgA2) reached maximum values during adulthood. PCs were already detected in newborns, increasing in number until 6 to 11 months for IgM, IgG1, IgG2, IgG3, IgA1, and IgA2; until 2 to 4 years for IgD; and until 5 to 9 years for IgG4 and decreasing thereafter. For most IgH isotypes (except IgD and IgG4), maximum plasma levels were reached after PC and MBC counts peaked. Conclusions: PC counts reach maximum values early in life, followed by MBC counts and plasma IgH isotypes. Importantly, IgH isotypes from different IGHC gene blocks show different patterns, probably reflecting consecutive cycles of IgH isotype switch recombination through life.pt_PT
dc.description.sponsorshipE.B. was supported by a grant from Junta de Castilla y León (Fondo Social Europeo, ORDEN EDU/346/2013, Valladolid, Spain). This work was supported by the CB16/12/00400 grant (CIBERONC, Instituto de Salud Carlos III, Ministerio de Economía y Competitividad, Madrid, Spain, and FONDOS FEDER) and the FIS PI12/00905-FEDER grant from the Fondo de Investigaciones Sanitarias of Instituto de Salud Carlos III (Madrid, Spain). O.P. and T.K. were supported by the Ministry of Education, Youth and Sports (NPU I no. LO1604 and 15-28541A). The coordination and innovation processes of this study were supported by the EuroFlow Consortium.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationJ Allergy Clin Immunol. 2018 Jun;141(6):2208-2219.e16pt_PT
dc.identifier.doi10.1016/j.jaci.2018.02.017pt_PT
dc.identifier.eissn1097-6825
dc.identifier.issn0091-6749
dc.identifier.urihttp://hdl.handle.net/10451/53064
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/journal/journal-of-allergy-and-clinical-immunologypt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectIgH isotypept_PT
dc.subjectImmunoglobulinspt_PT
dc.subjectAge-related valuespt_PT
dc.subjectFlow cytometrypt_PT
dc.subjectMemory B cellspt_PT
dc.subjectNormal B cellspt_PT
dc.subjectPlasma cellspt_PT
dc.subjectReference rangespt_PT
dc.subjectSubclasspt_PT
dc.titleAge-associated distribution of normal B-cell and plasma cell subsets in peripheral bloodpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage2219.e16pt_PT
oaire.citation.issue6pt_PT
oaire.citation.startPage2208pt_PT
oaire.citation.titleJournal of Allergy and Clinical Immunologypt_PT
oaire.citation.volume141pt_PT
person.familyNameCaetano
person.familyNameSousa
person.givenNameAna
person.givenNameAna E.
person.identifier61187
person.identifier.ciencia-idCE1E-9913-5BFB
person.identifier.ciencia-idD015-D3A2-992D
person.identifier.orcid0000-0002-1626-4364
person.identifier.orcid0000-0002-4618-9799
person.identifier.ridL-2642-2014
person.identifier.scopus-author-id7202484563
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication6e3ee8ec-15fa-4624-9d9d-14bf01a95ca9
relation.isAuthorOfPublication932aed1d-ffa8-494d-b958-43289d2b9cec
relation.isAuthorOfPublication.latestForDiscovery932aed1d-ffa8-494d-b958-43289d2b9cec

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