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Evidence for the dissemination to humans of Methicillin-Resistant Staphylococcus aureus ST398 through the pork production chain : a study in a portuguese slaughterhouse

2020-11-29Journal article Open access
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dc.contributor.authorBouchami, Ons
dc.contributor.authorFraqueza, M. J.
dc.contributor.authorFaria, Nuno Alexandre
dc.contributor.authorAlves, Valquíria
dc.contributor.authorLawal, Opeyemi Uwangbaoje
dc.contributor.authorLencastre, Herminia de
dc.contributor.authorMiragaia, Maria
dc.date.accessioned2022-04-19T22:47:01Z
dc.date.available2022-04-19T22:47:01Z
dc.date.issued2020-11-29
dc.descriptionResearch Areas: Microbiologypt_PT
dc.description.abstractLivestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) ST398 was recovered from infections in humans exposed to animals, raising public health concerns. However, contact with food producing chain as a means of transmission of LA-MRSA to humans remains poorly understood. We aimed to assess if pork production chain is a source of MRSA ST398 for human colonization and infection. MRSA from live pigs, meat, the environment, and slaughterhouse workers were analyzed by Pulsed-Field Gel Electrophoresis (PFGE), spa, MLST typing, SNPs and for antibiotic resistance and virulence gene profiles. We compared core and accessory genomes of MRSA ST398 isolated from slaughterhouse and hospital. We detected MRSA ST398 (t011, t108, t1451) along the entire pork production chain (live pigs: 60%; equipment: 38%; meat: 23%) and in workers (40%). All MRSA ST398 were multidrug resistant, and the majority carried genes encoding biocide resistance and enterotoxins. We found 23 cross-transmission events between live pigs, meat, and workers (6–55 SNPs). MRSA ST398 from infection and slaughterhouse environment belonged to the same clonal type (ST398, t011, SCCmec V), but differed in 321–378 SNPs. Pork production chain can be a source of MRSA ST398 for colonization of human slaughterhouse workers, which can represent a risk of subsequent meat contamination and human infection.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationBouchami O, Fraqueza MJ, Faria NA, Alves V, Lawal OU, Lencastre H, Miragaia M. 2020. Evidence for the dissemination to humans of Methicillin-Resistant Staphylococcus aureus ST398 through the pork production chain : a study in a portuguese slaughterhouse. Microorganisms, 8(12):1892. DOI 10.3390/microorganisms8121892pt_PT
dc.identifier.doi10.3390/microorganisms8121892pt_PT
dc.identifier.eissn2076-2607
dc.identifier.urihttp://hdl.handle.net/10400.5/24117
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationLISBOA-01-0145-FEDER007660pt_PT
dc.relationLISBOA-01-0145-FEDER-016417pt_PT
dc.relationApplied Molecular Biosciences Unit
dc.relation.publisherversionhttps://www.mdpi.com/2076-2607/8/12/1892pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectMRSApt_PT
dc.subjectST398pt_PT
dc.subjectSlaughterhousept_PT
dc.subjectPork chain productionpt_PT
dc.subjectTransmissionpt_PT
dc.subjectInvasive diseasept_PT
dc.titleEvidence for the dissemination to humans of Methicillin-Resistant Staphylococcus aureus ST398 through the pork production chain : a study in a portuguese slaughterhousept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleApplied Molecular Biosciences Unit
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F98317%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FFIS-NAN%2F0117%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FCVT-CVT%2F29510%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FMulti%2F04378%2F2019/PT
oaire.citation.conferencePlaceBasel, Switzerlandpt_PT
oaire.citation.titleMicroorganismspt_PT
oaire.citation.volume8(12):1892pt_PT
oaire.fundingStreamSFRH
oaire.fundingStream3599-PPCDT
oaire.fundingStream3599-PPCDT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameFraqueza
person.givenNameMaria João
person.identifierM-8503-2013
person.identifier.ciencia-idCA18-CCF2-652B
person.identifier.orcid0000-0002-8388-0511
person.identifier.scopus-author-id22953692100
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication247d02c1-b9b9-4c96-852b-d03f7bb0a6dd
relation.isAuthorOfPublication.latestForDiscovery247d02c1-b9b9-4c96-852b-d03f7bb0a6dd
relation.isProjectOfPublicationabec9469-646d-40f8-8ab6-2c7f83402f48
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