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Fighting S. aureus catheter-related infections with sophorolipids: electing an antiadhesive strategy or a release one?

2021Journal article Restricted access
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dc.contributor.authorMendes, Rita M.
dc.contributor.authorFrancisco, Ana P.
dc.contributor.authorCarvalho, Filomena Almeida
dc.contributor.authorDardouri, Maissa
dc.contributor.authorCosta, Bruna
dc.contributor.authorBettencourt, Ana F.
dc.contributor.authorCosta, Judite
dc.contributor.authorGonçalves, Lidia
dc.contributor.authorCosta, Fabíola
dc.contributor.authorRibeiro, Isabel A.C.
dc.date.accessioned2021-09-17T15:46:27Z
dc.date.available2021-09-17T15:46:27Z
dc.date.issued2021
dc.description© 2021 Elsevier B.V. All rights reserved.pt_PT
dc.description.abstractStaphylococcus aureus medical devices related-infections, such as blood stream catheter are of major concern. Their prevention is compulsory and strategies, not prone to the development of resistance, to prevent S. aureus biofilms on catheter surfaces (e.g. silicone) are needed. In this work two different approaches using sophorolipids were studied to prevent S. aureus biofilm formation on medical grade silicone: i) an antiadhesive strategy through covalent bond of sophorolipids to the surface; ii) and a release strategy using isolated most active sophorolipids. Sophorolipids produced by Starmerella bombicola, were characterized by UHPLC-MS and RMN, purified by automatic flash chromatography and tested for their antimicrobial activity towards S. aureus. Highest antimicrobial activity was observed for C18:0 and C18:1 diacetylated lactonic sophorolipids showing a MIC of 50 μg mL-1. Surface modification with acidic or lactonic sophorolipids when evaluating the anti-adhesive or release strategy, respectively, was confirmed by contact angle, FTIR-ATR and AFM analysis. When using a mixture of acidic sophorolipids covalently bonded to silicone surface as antiadhesive strategy cytocompatible surfaces were obtained and a reduction of 90 % on biofilm formation was observed. Nevertheless, if a release strategy is adopted with purified lactonic sophorolipids a higher effect is achieved. Most promising compound was C18:1 diacateylated lactonic sophorolipid that showed no cellular viability reduction when a concentration of 1.5 mg mL-1 was selected and a reduction on biofilm around 5 log units. Results reinforce the applicability of these antimicrobial biosurfactants on preventing biofilms and disclose that their antimicrobial effect is imperative when comparing to their antiadhesive properties.pt_PT
dc.description.sponsorshipThe authors thank Fundação para a Ciência e Tecnologia (FCT) for the financial support under Projects PTDC/BTM-SAL/29335/2017, UIDB/04138/2020, UIDP/04138/2020 (iMed.ULisboa) and Portugal 2020 for the Portuguese Mass Spectrometry Network (Rede Nacional de Espectrometria de Massa RNEM; LISBOA 01 0145 FEDER 402 022125).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationColloids Surf B Biointerfaces. 2021 Aug 23;208:112057pt_PT
dc.identifier.doi10.1016/j.colsurfb.2021.112057pt_PT
dc.identifier.eissn1873-4367
dc.identifier.issn0927-7765
dc.identifier.urihttp://hdl.handle.net/10451/49540
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/journal/colloids-and-surfaces-b-biointerfacespt_PT
dc.subjectAnti-adhesivept_PT
dc.subjectAntibacterial surfacept_PT
dc.subjectBiofilmpt_PT
dc.subjectReleasept_PT
dc.subjectS. aureuspt_PT
dc.subjectSelf-assembling monolayerspt_PT
dc.titleFighting S. aureus catheter-related infections with sophorolipids: electing an antiadhesive strategy or a release one?pt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/150523/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/157522/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/157836/PT
oaire.citation.titleColloids and Surfaces B: Biointerfacespt_PT
oaire.citation.volume208pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameCarvalho
person.givenNameFilomena
person.identifier.ciencia-id4615-4AEC-8D91
person.identifier.orcid0000-0001-6088-3894
person.identifier.ridM-9202-2013
person.identifier.scopus-author-id7103069694
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublication.latestForDiscovery547cb993-880c-4608-b1e0-493ad469eecb
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