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Systematic Modification and Evaluation of Enzyme-Loaded Chitosan Nanoparticles

2021-07-26Journal article Open access
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dc.contributor.authorLino, Paulo Roque
dc.contributor.authorLeandro, João
dc.contributor.authorFigueiredo, Lara
dc.contributor.authorAmaro, Mariana P.
dc.contributor.authorGonçalves, Lídia
dc.contributor.authorLeandro, Paula
dc.contributor.authorAlmeida, António José
dc.date.accessioned2023-09-14T11:19:57Z
dc.date.available2023-09-14T11:19:57Z
dc.date.issued2021-07-26
dc.date.updated2023-02-27T15:42:55Z
dc.description.abstractPolymeric-based nano drug delivery systems have been widely exploited to overcome protein instability during formulation. Presently, a diverse range of polymeric agents can be used, among which polysaccharides, such as chitosan (CS), hyaluronic acid (HA) and cyclodextrins (CDs), are included. Due to its unique biological and physicochemical properties, CS is one of the most used polysaccharides for development of protein delivery systems. However, CS has been described as potentially immunogenic. By envisaging a biosafe cytocompatible and haemocompatible profile, this paper reports the systematic development of a delivery system based on CS and derived with HA and CDs to nanoencapsulate the model human phenylalanine hydroxylase (hPAH) through ionotropic gelation with tripolyphosphate (TPP), while maintaining protein stability and enzyme activity. By merging the combined set of biopolymers, we were able to effectively entrap hPAH within CS nanoparticles with improvements in hPAH stability and the maintenance of functional activity, while simultaneously achieving strict control of the formulation process. Detailed characterization of the developed nanoparticulate systems showed that the lead formulations were internalized by hepatocytes (HepG2 cell line), did not reveal cell toxicity and presented a safe haemocompatible profile.pt_PT
dc.description.sponsorshipThis work was supported by FEDER and Fundação para a Ciência e a Tecnologia, I. P. through national funds: Projects UIDB/04138/2020 and UIDP/04138/2020, research projects PTDC/QUI/64023/2006 and PTDC/EBB-BIO/101237/2008; and grants SFRH/BSAB/1210/2011 (to A.J.A.) and SFRH/BD/47946/2008 (to P.R.L.). This work also received funding from the National PKU Alliance, USA.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationLino PR, Leandro J, Figueiredo L, Amaro MP, Gonçalves LMD, Leandro P, et al. Systematic modification and evaluation of enzyme-loaded chitosan nanoparticles. International Journal of Molecular Sciences [Internet]. janeiro de 2021;22(15):7987. Disponível em: https://www.mdpi.com/1422-0067/22/15/7987pt_PT
dc.identifier.doi10.3390/ijms22157987pt_PT
dc.identifier.slugcv-prod-3147553
dc.identifier.urihttp://hdl.handle.net/10451/59288
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationPTDC/QUI/64023/2006pt_PT
dc.relationSFRH/BSAB/1210/2011pt_PT
dc.relationSFRH/BD/47946/2008pt_PT
dc.relation.publisherversionhttps://www.mdpi.com/1422-0067/22/15/7987pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectchitosanpt_PT
dc.subjecthyaluronic acidpt_PT
dc.subjectcyclodextrinspt_PT
dc.subjectbiocompatibility modulationpt_PT
dc.subjectenzyme deliverypt_PT
dc.subjectcomplement systempt_PT
dc.subjecthaemocompatibilitypt_PT
dc.subjectphenylketonuriapt_PT
dc.subjecthuman phenylalanine hydroxylasept_PT
dc.subjectnanoparticlespt_PT
dc.titleSystematic Modification and Evaluation of Enzyme-Loaded Chitosan Nanoparticlespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FEBB-BIO%2F101237%2F2008/PT
oaire.citation.issue15pt_PT
oaire.citation.startPage7987pt_PT
oaire.citation.titleInternational Journal of Molecular Sciencespt_PT
oaire.citation.volume22pt_PT
oaire.fundingStream3599-PPCDT
person.familyNameLino
person.familyNameDiogo Gonçalves
person.familyNameLeandro
person.familyNameLeitão das Neves Almeida
person.givenNamePaulo
person.givenNameLídia Maria
person.givenNamePaula
person.givenNameAntónio José
person.identifierI-6590-2012
person.identifier.ciencia-id7211-22BA-86AD
person.identifier.ciencia-id1610-4815-076E
person.identifier.ciencia-id8312-F853-C47A
person.identifier.orcid0000-0003-4221-4499
person.identifier.orcid0000-0002-6799-2740
person.identifier.orcid0000-0002-2946-9342
person.identifier.orcid0000-0002-7807-4726
person.identifier.ridN-2179-2015
person.identifier.ridA-8323-2014
person.identifier.scopus-author-id56427809800
person.identifier.scopus-author-id6602522134
person.identifier.scopus-author-id57195052432
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.cv.cienciaid7211-22BA-86AD | Lídia Maria Diogo Gonçalves
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicatione73e0d2c-d5e2-4f57-9fa4-7be4f0903d9e
relation.isAuthorOfPublication3265148d-dc2d-4601-a832-2e72f7be5662
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relation.isAuthorOfPublicationdab457ee-7a1d-4c3d-a906-1e8df92fffd6
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