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Understanding the impact of drought in Coffea genotypes: transcriptomic analysis supports a common high resilience to moderate water deficit but a genotype dependent sensitivity to severe water deficit
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Orientador(es)
Resumo(s)
Water scarcity is the most significant factor limiting coffee production, although some
cultivars can still have important drought tolerance. This study analyzed leaf transcriptomes of
two coffee cultivars with contrasting physiological responses, Coffea canephora cv. CL153 and Coffea.
arabica cv. Icatu, subjected to moderate (MWD) or severe water deficits (SWD). We found that
MWD had a low impact compared with SWD, where 10% of all genes in Icatu and 17% in CL153
reacted to drought, being mainly down-regulated upon stress. Drought triggered a genotype-specific
response involving the up-regulation of reticuline oxidase genes in CL153 and heat shock proteins
in Icatu. Responsiveness to drought also included desiccation protectant genes, but primarily,
aspartic proteases, especially in CL153. A total of 83 Transcription Factors were found engaged in
response to drought, mainly up-regulated, especially under SWD. Together with the enrollment
of 49 phosphatases and 272 protein kinases, results suggest the involvement of ABA-signaling
processes in drought acclimation. The integration of these findings with complementing physiological
and biochemical studies reveals that both genotypes are more resilient to moderate drought than
previously thought and suggests the existence of post-transcriptional mechanisms modulating the
response to drought
Descrição
Palavras-chave
ABA signaling climate changes coffee drought functional analysis leaf RNAseq transcription factors
Contexto Educativo
Citação
Fernandes, I.; Marques, I.; Paulo, O.S.; Batista, D.; Partelli, F.L.; Lidon, F.C.; DaMatta, F.M.; Ramalho, J.C.; Ribeiro-Barros, A.I. Understanding the Impact of Drought in Coffea Genotypes: Transcriptomic Analysis Supports a Common High Resilience to ModerateWater Deficit but a Genotype Dependent Sensitivity to SevereWater Deficit. Agronomy 2021, 11, 2255
Editora
MDPI