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Freshwater fish community assessment using eDNA metabarcoding vs. capture-based methods: Differences in efficiency and resolution coupled to habitat and ecology

2025-06Artigo científico Acesso embargado
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dc.contributor.authorCurto, Manuel
dc.contributor.authorBatista, Sofia
dc.contributor.authorSantos, Carlos D.
dc.contributor.authorRibeiro, Filipe
dc.contributor.authorNogueira, Sofia
dc.contributor.authorRibeiro, Diogo
dc.contributor.authorPrindle, Benjamin
dc.contributor.authorLicari, Daniel
dc.contributor.authorRiccioni, Giulia
dc.contributor.authorDias, Diogo
dc.contributor.authorPina-Martins, Francisco
dc.contributor.authorJentoft, Sissel
dc.contributor.authorVeríssimo, Ana
dc.contributor.authorAlves, Maria Judite
dc.contributor.authorGante, Hugo F.
dc.date.accessioned2025-04-01T11:40:58Z
dc.date.embargo2027-05-12
dc.date.issued2025-06
dc.description.abstractEnvironmental DNA (eDNA) metabarcoding has revolutionized ecological and environmental research by describing communities without relying on direct observations, making it a powerful, non-invasive, and cost-effective tool in biodiversity monitoring. However, the adoption of eDNA as a standard protocol in long-term monitoring programs, which have traditionally relied on capture-based methods, presents challenges in terms of data comparability. Here, we compared freshwater fish communities assessed through eDNA metabarcoding and electrofishing, across 35 sampling sites in the lower Tagus River basin, Portugal. For the majority of species or species-groups analysed individually (13 out of 17), a significant correspondence was observed between electrofishing and eDNA metabarcoding detections. A weaker correspondence was found between the number of specimens captured by electrofishing with the number of eDNA metabarcoding reads, with seven out of 13 taxa showing significant relationships. Species richness estimates based on the two methods were very similar at the basin level. The methods yielded significantly different species compositions, although these differences were driven by samples collected in the Tagus main channel, which is wider and has higher flow rates than tributaries. Benthic and shoreline fish communities showed similar species composition in the two methods, but this was not the case for pelagic communities, probably due to the higher water turnover of the pelagic zone and electrofishing inefficiency. Our results highlight the high potential of eDNA metabarcoding as a complementary method to electrofishing for freshwater fish monitoring, though further validation is needed to assess biases related to site-specific hydrological conditions and the ecology of the target species.pt_PT
dc.description.versioninfo:eu-repo/semantics/acceptedVersionpt_PT
dc.identifier.doi10.1016/j.envres.2025.121238pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.5/99896
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationMarine and Environmental Sciences Centre
dc.relationAquatic Research Infrastructure Network
dc.relationTeaming to Upgrade to Excellence in Environmental Biology, Ecosystem Research and AgroBiodiversity
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S001393512500489X?dgcid=coauthorpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.titleFreshwater fish community assessment using eDNA metabarcoding vs. capture-based methods: Differences in efficiency and resolution coupled to habitat and ecologypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMarine and Environmental Sciences Centre
oaire.awardTitleAquatic Research Infrastructure Network
oaire.awardTitleTeaming to Upgrade to Excellence in Environmental Biology, Ecosystem Research and AgroBiodiversity
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBIA-CBI%2F31644%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/Concurso de avaliação no âmbito do Programa Plurianual de Financiamento de Unidades de I&D (2017%2F2018) - Financiamento Programático/UIDP%2F04292%2F2020/PT
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oaire.awardURIinfo:eu-repo/grantAgreement/FCT/Concurso de avaliação no âmbito do Programa Plurianual de Financiamento de Unidades de I&D (2017%2F2018) - Financiamento Programático/UIDP%2F00329%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/Concurso de avaliação no âmbito do Programa Plurianual de Financiamento de Unidades de I&D (2017%2F2018) - Financiamento Programático/UIDP%2F50027%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0069%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/EC/H2020/857251/EU
oaire.citation.startPage121238pt_PT
oaire.citation.titleEnvironmental Researchpt_PT
oaire.citation.volume274pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStreamConcurso de avaliação no âmbito do Programa Plurianual de Financiamento de Unidades de I&D (2017/2018) - Financiamento Programático
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamConcurso de avaliação no âmbito do Programa Plurianual de Financiamento de Unidades de I&D (2017/2018) - Financiamento Programático
oaire.fundingStreamConcurso de avaliação no âmbito do Programa Plurianual de Financiamento de Unidades de I&D (2017/2018) - Financiamento Programático
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamH2020
person.familyNamePina Martins
person.givenNameFrancisco
person.identifier.ciencia-id1E1C-2D9B-5338
person.identifier.orcid0000-0003-1836-397X
person.identifier.scopus-author-id25630967600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
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project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameEuropean Commission
rcaap.embargofctO tipo de licença envolvido com a editora desta revista, quando não envolve pagamento (APC) apenas permite acesso em repositório público do formato 'aceite' (i.e não editado e paginado pela editora), após 24 meses da sua publicação final.pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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