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Molecular detection of Helicobacter pyloriand its genotypic antimicrobial resistance patterns in dyspeptic Mozambican patients

2023-06-20Journal article Restricted access
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dc.contributor.authorIsmail, Muhammad Ahamed
dc.contributor.authorMajaliwa, Nashon D.
dc.contributor.authorVale, Filipa F.
dc.contributor.authorCumbana, Roqueia
dc.contributor.authorSumbana, José J.
dc.contributor.authorMuchongo, Arsénio
dc.contributor.authorNassone, Ema
dc.contributor.authorLoforte, Michella
dc.contributor.authorMondlane, Liana
dc.contributor.authorBotão, Edília
dc.contributor.authorTaviani, Elisa
dc.contributor.authorCarrilho, Carla
dc.contributor.authorVítor, Jorge M. B.
dc.contributor.authorSacarlal, Jahit
dc.date.accessioned2023-08-24T15:44:52Z
dc.date.available2023-08-24T15:44:52Z
dc.date.issued2023-06-20
dc.date.updated2023-07-10T13:36:02Z
dc.description.abstractBackground Helicobacter pylori strains show a high level of genotypic diversity and express several genes that contribute to their pathogenicity and resistance. In Mozambique, there is lack of information regarding its resistance pattern to antibiotics. In this study, we aimed to investigate the prevalence of H. pylori and its genotypic resistance to clarithromycin, metronidazole, and fluoroquinolones in Mozambican dyspeptic patients. Since appropriate eradication should be based on the local resistance rate, our data will guide clinicians in choosing the best drugs for the effective treatment of H. pylori-infected patients. Methods This is a cross-sectional descriptive study conducted between June 2017 and June 2020, in which 171 dyspeptic patients were recruited, and through upper gastrointestinal endoscopy, gastric biopsies were collected from those patients. Polymerase chain reaction was performed for the detection of H. pylori and its resistance mechanisms to clarithromycin (23S rRNA), metronidazole (rdxA), and fluoroquinolones (gyrA); mutations conferring resistance to these antibiotics were investigated by sequencing 23S rRNA, rdxA, and gyrA genes. Results Of the 171 samples tested, H. pylori was detected in 56.1% (96/171). The clarithromycin resistance rate was 10.4% (the responsible mutations were A2142G and A2143G), the metronidazole resistance rate was 55.2% (4 types of mutations responsible for metronidazole resistance were identified which include, D59N, R90K, H97T, and A118T. However, in many cases, they appeared in combination, with D59N + R90K + A118T being the most frequent combination), and the fluoroquinolones resistance rate was 20% (the responsible mutations were N87I and D91G). Conclusion H. pylori infection remains common in dyspeptic Mozambican patients. High resistance to metronidazole and fluoroquinolones requires continuous monitoring of antibiotic resistance and adaptation of therapy to eradicate this infection.pt_PT
dc.description.sponsorshipFundo Nacional de Investigação (FNI) and Agência Italiana de Cooperação para o Desenvolvimento through Biotechnology Center (Universidade Eduardo Mondlane), Mozambique. NDM was a recipient of a master fellowship from Instituto Gulbenkian de Ciência of Fundação Calouste Gulbenkian, Portugal. FFV is a recipient of a project grant (PTDC/BTM-TEC/3238/2020) that partially supported the molecular methods. The work has been partially supported by National Funds from Fundação para a Ciência e a Tecnologia, projects UIDB/04138/2020 and UIDP/04138/2020.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationIsmail M, Majaliwa ND, Vale FF, Cumbana R, Sumbana JJ, Muchongo A, et al. Molecular detection of Helicobacter pylori and its genotypic antimicrobial resistance patterns in dyspeptic Mozambican patients. Helicobacter [Internet]. agosto de 2023;28(4):e13000. Disponível em: https://onlinelibrary.wiley.com/doi/10.1111/hel.13000pt_PT
dc.identifier.doi10.1111/hel.13000pt_PT
dc.identifier.issn1083-4389
dc.identifier.issn1523-5378
dc.identifier.slugcv-prod-3300734
dc.identifier.urihttp://hdl.handle.net/10451/59006
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherWileypt_PT
dc.relationResearch Institute for Medicines
dc.relationResearch Institute for Medicines
dc.relation.publisherversionhttps://onlinelibrary.wiley.com/doi/10.1111/hel.13000pt_PT
dc.subjectantibiotic resistancept_PT
dc.subjectHelicobacter pyloript_PT
dc.subjectMozambiquept_PT
dc.titleMolecular detection of Helicobacter pyloriand its genotypic antimicrobial resistance patterns in dyspeptic Mozambican patientspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleResearch Institute for Medicines
oaire.awardTitleResearch Institute for Medicines
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBTM-TEC%2F3238%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04138%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04138%2F2020/PT
oaire.citation.issue4pt_PT
oaire.citation.startPagee13000pt_PT
oaire.citation.titleHelicobacterpt_PT
oaire.citation.volume28pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameIsmail
person.familyNameVale
person.familyNameVítor
person.familyNameSacarlal
person.givenNameMuhammad Ahamed
person.givenNameFilipa
person.givenNameJorge
person.givenNameJahit
person.identifier.ciencia-id4718-00EF-4BF0
person.identifier.ciencia-idFF19-99F4-3964
person.identifier.orcid0000-0002-4625-9865
person.identifier.orcid0000-0003-4635-0105
person.identifier.orcid0000-0001-6486-3444
person.identifier.orcid0000-0003-1059-217X
person.identifier.ridC-3570-2013
person.identifier.ridM-3279-2013
person.identifier.ridR-3172-2016
person.identifier.scopus-author-id17136133700
person.identifier.scopus-author-id7801493621
person.identifier.scopus-author-id9845325600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.cv.cienciaidFF19-99F4-3964 | Jorge Manuel Barreto Vítor
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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