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Identificação de potenciais marcadores glicosídicos associados a alterações malignas em pulmão
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O Cancro de Pulmão (CP) está entre as principais causas de morte no mundo representando 15% de todos os cancros. Há quatro tipos mais comuns de CP e sabe-se que apresentam comportamento e agressividade distintos. A expressão de glicosilação aberrante por parte das células de CP tem sido descrita. Contudo a sua correlação com o prognóstico clínico e agressividade da doença ainda não está esclarecida. Este estudo teve como objetivo geral contribuir para a identificação de novos marcadores de prognóstico de CP. Em particular, neste trabalho pretendeu-se: Estabelecer um biobanco de vários tipos de amostras de tecido provenientes de doentes com CP, Estabelecer ensaios piloto com vista à validação do biobanco e à otimização de protocolos de estudo da expressão genética no tipo de amostras, que compõem o biobanco, Analisar modelos celulares para estabelecer uma correlação entre a expressão de determinados genes e a expressão de estruturas glicosídicas. O biobanco estabelecido consistiu em 58 amostras de aspirados ganglionares, 105 amostras de líquido pleural e amostras de tecido de tumor e tecido sem tumor de 43 doentes submetidos a biopsia. Em termos de expressão genética, verificou-se significativas alterações da expressão de genes envolvidos na expressão de estruturas glicosídicas, nomeadamente estruturas contendo o açúcar fucose e de genes envolvidos na resposta inflamatória. Presume-se que quando for possível reunir a totalidade dos dados clínicos, tais como estadiamento e tempo de vida, em conjunto com as análises dos diversos tipos de amostras seja possível encontrar diferenças significativas na expressão dos genes e que estas diferenças contribuam para estabelecer um valor prognóstico em doentes com cancro de pulmão.
Lung cancer (LC) is among the leading causes of death in the world representing 15% of all cancers. There are four common types of LC and it is known that exhibit different behavior and aggressiveness. The aberrant glycosylation expression on the part of LC cells has been described. However its correlation with clinical prognosis and aggressiveness of the disease is not yet clarified. This study had as general goal to contribute for the identification of new prognostic markers of LC, in particular, this study was intended to: Establish a biobank of various types of tissue samples from patients with LC, Establish pilot studies for the biobank validation and optimization study protocols of gene expression in type of samples, which make up the biobank, Analyze cellular models to establish a correlation between the expression of certain genes and expression of glycosidic linkages. The biobank established consisted of 58 samples of lymph nodes aspirates, 105 pleural effusion samples and tumor tissue samples and tumor free tissue of 43 patients undergoing biopsy. In terms of gene expression, there have been significant changes in the expression of genes involved in the expression of glycosidic structures, including structures containing sugar fucose and genes involved in the inflammatory response. It is assumed that when possible to gather all of the clinical data, such as staging and lifetime, together with analyses of various types of samples, is possible to find significant differences in gene expression. These differences will contribute to establish a prognostic value in patients with lung cancer.
Lung cancer (LC) is among the leading causes of death in the world representing 15% of all cancers. There are four common types of LC and it is known that exhibit different behavior and aggressiveness. The aberrant glycosylation expression on the part of LC cells has been described. However its correlation with clinical prognosis and aggressiveness of the disease is not yet clarified. This study had as general goal to contribute for the identification of new prognostic markers of LC, in particular, this study was intended to: Establish a biobank of various types of tissue samples from patients with LC, Establish pilot studies for the biobank validation and optimization study protocols of gene expression in type of samples, which make up the biobank, Analyze cellular models to establish a correlation between the expression of certain genes and expression of glycosidic linkages. The biobank established consisted of 58 samples of lymph nodes aspirates, 105 pleural effusion samples and tumor tissue samples and tumor free tissue of 43 patients undergoing biopsy. In terms of gene expression, there have been significant changes in the expression of genes involved in the expression of glycosidic structures, including structures containing sugar fucose and genes involved in the inflammatory response. It is assumed that when possible to gather all of the clinical data, such as staging and lifetime, together with analyses of various types of samples, is possible to find significant differences in gene expression. These differences will contribute to establish a prognostic value in patients with lung cancer.
Descrição
Tese de mestrado. Biologia (Biologia Molecular e Genética). Universidade de Lisboa, Faculdade de Ciências, 2012
Palavras-chave
Cancro do pulmão Glicosilação Adenocarcinoma Marcadores genéticos Expressão génica Teses de mestrado - 2012