A carregar...
Publicação
Red blood cell membrane integrity in primary open angle glaucoma : ex vivo and in vitro studies
| Nome: | Descrição: | Tamanho: | Formato: | |
|---|---|---|---|---|
| 187.83 KB | Adobe PDF |
Orientador(es)
Resumo(s)
Purpose There is increasing evidence that abnormal perfusion of the optic nerve head is
an important factor involved in the pathophysiology of glaucoma. Transport and distribution of oxygen to the tissues takes place through the erythrocyte membrane. Red blood cell (RBC) acetylcholinesterase (AChE) is a marker of RBC membrane integrity. The aim of this study was to find out whether RBC membrane integrity is preserved in primary open angle glaucoma (POAG), and whether it is modified by the use of topical timolol maleate and pilocarpine.
Method RBC AChE activity was determined ex vivo by Kaplan's spectrophotometric method in 19 POAG patients undergoing topical treatment for glaucoma with timolol, pilocarpine or a combination of the two drugs, and compared with that in 20 controls. To assess the effect of antiglaucomatous therapy in our findings, we carried out an in vitro study in 26 non-glaucomatous patients in which we measured RBC AChE activity after incubation of blood with either timolol maleate, pilocarpine or a combination of the two drugs, using the same spectrophotometric method.
Results There was a significant increase in RBC AChE enzyme activity in POAG patients compared with the control group (p < 0.002). However, timolol and pilocarpine, individually or in combination, have the opposite effect, significantly decreasing RBC AChE activity (p < 0.05).
Conclusion This change in RBC AChE enzyme activity could suggest alterations in RBC membrane integrity in primary open angle glaucoma. Whether or not this finding has implications regarding the microcirculation at the optic nerve head needs to be investigated further.
Descrição
This work was presented as a poster at the Tenth Anniversary Congress of the Royal College of Ophthalmologists, Glasgow 1998
© 1999 Royal College of Ophthalmologists
© 1999 Royal College of Ophthalmologists
Palavras-chave
Acetylcholinesterase Glaucoma Pilocarpine Red blood cell Timolol
Contexto Educativo
Citação
Eye (1999) 13, 101-103