Name: | Description: | Size: | Format: | |
---|---|---|---|---|
775.12 KB | Adobe PDF |
Advisor(s)
Abstract(s)
Lactic acid bacteria (LAB) have been documented as potential vitamin B12 producers and
may constitute an exogenous source of cobalamin for the microalga Chlorella vulgaris, which has
been described as being able to perform vitamin uptake. Hence, there is an interest in discovering
novel B12-producing probiotic LAB. Therefore, the purpose of the current work was to perform
a phenotype–genotype analysis of the vitamin B12 biosynthesis capacity of LAB isolated from C.
vulgaris bioreactors, and investigate their probiotic potential. Among the selected strains, Lactococcus
lactis E32, Levilactobacillus brevis G31, and Pediococcus pentosaceus L51 demonstrated vitamin B12
biosynthesis capacity, with the latter producing the highest (28.19 ± 2.27 pg mL−1
). The genomic
analysis confirmed the presence of pivotal genes involved in different steps of the biosynthetic
pathway (hemL, cbiT, cobC, and cobD). Notably, P. pentosaceus L51 was the only strain harboring
cobA, pduU, and pduV genes, which may provide evidence for the presence of the cobalamin operon.
All strains demonstrated the capability to withstand harsh gastrointestinal conditions, although P.
pentosaceus L51 was more resilient. The potential for de novo cobalamin biosynthesis and remarkable
probiotic features highlighted that P. pentosaceus L51 may be considered the most promising candidate
strain for developing high-content vitamin B12 formulations.
Description
Keywords
cobalamin B12 biosynthesis B12-producing probiotic lactic acid bacteria C. vulgaris
Pedagogical Context
Citation
Publisher
MDPI