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Ctdnep1 and Eps8L2 regulate dorsal actin cables for nuclear positioning during cell migration

2021Journal article Open access
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dc.contributor.authorCalero-Cuenca, Francisco J.
dc.contributor.authorOsorio, Daniel S.
dc.contributor.authorCarvalho-Marques, Sofia
dc.contributor.authorSridhara, Sree Rama Chaitanya
dc.contributor.authorOliveira, Luis M.
dc.contributor.authorJiao, Yue
dc.contributor.authorDiaz, Jheimmy
dc.contributor.authorJanota, Cátia
dc.contributor.authorCadot, Bruno
dc.contributor.authorGomes, Edgar
dc.date.accessioned2021-07-28T14:44:13Z
dc.date.available2021-07-28T14:44:13Z
dc.date.issued2021
dc.description© 2021 The Author(s). Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0).pt_PT
dc.description.abstractCells actively position their nuclei within the cytoplasm for multiple cellular and physiological functions.1-3 Consequently, nuclear mispositioning is usually associated with cell dysfunction and disease, from muscular disorders to cancer metastasis.4-7 Different cell types position their nuclei away from the leading edge during cell migration.8-11 In migrating fibroblasts, nuclear positioning is driven by an actin retrograde flow originated at the leading edge that drives dorsal actin cables away from the leading edge. The dorsal actin cables connect to the nuclear envelope by the linker of nucleoskeleton and cytoskeleton (LINC) complex on transmembrane actin-associated nuclear (TAN) lines.12-14 Dorsal actin cables are required for the formation of TAN lines. How dorsal actin cables are organized to promote TAN lines formation is unknown. Here, we report a role for Ctdnep1/Dullard, a nuclear envelope phosphatase,15-22 and the actin regulator Eps8L223-25 on nuclear positioning and cell migration. We demonstrate that Ctdnep1 and Eps8L2 directly interact, and this interaction is important for nuclear positioning and cell migration. We also show that Ctdnep1 and Eps8L2 are involved in the formation and thickness of dorsal actin cables required for TAN lines engagement during nuclear movement. We propose that Ctdnep1-Eps8L2 interaction regulates dorsal actin cables for nuclear movement during cell migration.pt_PT
dc.description.sponsorshipThis work was supported by the European Research Council (810207 and 617676); EMBO Installation (2758); Association pour la Recherche sur le Cancer; La Ligue Nacionale contre le Cancer; LISBOA-01-0145-FEDER-007391 project co-funded by FEDER, through POR Lisboa 2020 – Programa Operacional Regional de Lisboa, PORTUGAL 2020; and Fundação para a Ciência e a Tecnologia.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationCurr Biol. 2021 Apr 12;31(7):1521-1530.e8pt_PT
dc.identifier.doi10.1016/j.cub.2021.01.007pt_PT
dc.identifier.eissn1879-0445
dc.identifier.issn0960-9822
dc.identifier.urihttp://hdl.handle.net/10451/49194
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationLISBOA-01-0145-FEDER-007391pt_PT
dc.relationDefining the role of Arp2/3 complex diversity at multiple scales of biology
dc.relationPositioning the nucleus for cell migration and muscle fiber function
dc.relation.publisherversionhttps://www.sciencedirect.com/journal/current-biologypt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectCtdnep1pt_PT
dc.subjectEps8L2pt_PT
dc.subjectLINC Complexpt_PT
dc.subjectTAN linespt_PT
dc.subjectActinpt_PT
dc.subjectCell migrationpt_PT
dc.subjectNuclear positioningpt_PT
dc.titleCtdnep1 and Eps8L2 regulate dorsal actin cables for nuclear positioning during cell migrationpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleDefining the role of Arp2/3 complex diversity at multiple scales of biology
oaire.awardTitlePositioning the nucleus for cell migration and muscle fiber function
oaire.awardURIinfo:eu-repo/grantAgreement/EC/H2020/810207/EU
oaire.awardURIinfo:eu-repo/grantAgreement/EC/FP7/617676/EU
oaire.citation.endPage1530pt_PT
oaire.citation.issue7pt_PT
oaire.citation.startPage1521pt_PT
oaire.citation.titleCurrent Biologypt_PT
oaire.citation.volume31pt_PT
oaire.fundingStreamH2020
oaire.fundingStreamFP7
person.familyNameCalero-Cuenca
person.familyNameCarvalho-Marques
person.familyNameSridhara
person.familyNameJanota
person.familyNameGomes
person.givenNameFrancisco Javier
person.givenNameSofia
person.givenNameSree Rama Chaitanya
person.givenNameCátia
person.givenNameEdgar
person.identifier.ciencia-id8A10-487C-AD83
person.identifier.ciencia-id6215-9445-3042
person.identifier.orcid0000-0002-4212-3944
person.identifier.orcid0000-0002-8983-0531
person.identifier.orcid0000-0001-5219-2014
person.identifier.orcid0000-0003-2187-2361
person.identifier.orcid0000-0002-6941-4872
person.identifier.ridN-7059-2018
person.identifier.ridD-9000-2015
person.identifier.scopus-author-id7102464212
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.nameEuropean Commission
project.funder.nameEuropean Commission
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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