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5-Aza-20 -Deoxycytidine (5-Aza-dC, Decitabine) Inhibits Collagen Type I and III Expression in TGF-β1-Treated Equine Endometrial Fibroblasts

2023-03-30Artigo científico Acesso aberto
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dc.contributor.authorAlpoim-Moreira, J.
dc.contributor.authorSzóstek-Mioduchowska, A.
dc.contributor.authorSłyszewska, M.
dc.contributor.authorRebordão, M.R.
dc.contributor.authorSkarzynski, D.J.
dc.contributor.authorFerreira-Dias, Graça
dc.date.accessioned2023-05-21T21:21:58Z
dc.date.available2023-05-21T21:21:58Z
dc.date.issued2023-03-30
dc.descriptionResearch Areas: Agriculture ; Veterinary Sciences ; Zoologypt_PT
dc.description.abstractEndometrosis negatively affects endometrial function and fertility in mares, due to excessive deposition of type I (COL1) and type III (COL3) collagens. The pro-fibrotic transforming growth factor (TGF-β1) induces myofibroblast differentiation, characterized by α-smooth muscle actin (α-SMA) expression, and collagen synthesis. In humans, fibrosis has been linked to epigenetic mechanisms. To the best of our knowledge, this has not been described in mare endometrium. Therefore, this study aimed to investigate the in vitro epigenetic regulation in TGF-β1-treated mare endometrial fibroblasts and the use of 5-aza-20 -deoxycytidine (5-aza-dC), an epigenetic modifier, as a putative treatment option for endometrial fibrosis. Methods and Results: The in vitro effects of TGF-β1 and of 5-aza-dC on DNA methyltransferases (DNMT1, DNMT3A, and DNMT3B), COL1A1, COL3A1, and α-SMA transcripts were analyzed in endometrial fibroblasts, and COL1 and COL3 secretion in a co-culture medium. TGF-β1 upregulated DNMT3A transcripts and collagen secretion. In TGF-β1-treated endometrial fibroblasts, DNA methylation inhibitor 5-aza-dC decreased collagen transcripts and secretion, but not α-SMA transcripts. Conclusion: These findings suggest a possible role of epigenetic mechanisms during equine endometrial fibrogenesis. The in vitro effect of 5-aza-dC on collagen reduction in TGF-β1-treated fibroblasts highlights this epigenetic involvement. This may pave the way to different therapeutic approaches for endometrosispt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationAlpoim-Moreira J, Szóstek-Mioduchowska A, Słyszewska M, Rebordão MR, Skarzynski DJ, Ferreira-Dias G. 2023. 5-Aza-20 -Deoxycytidine (5-Aza-dC, Decitabine) Inhibits Collagen Type I and III Expression in TGF-β1-Treated Equine Endometrial Fibroblasts. Animals, 13(7):1212. DOI: https:// doi.org/10.3390/ani13071212pt_PT
dc.identifier.doi10.3390/ani13071212pt_PT
dc.identifier.issn2076-2615
dc.identifier.urihttp://hdl.handle.net/10400.5/27791
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relation2022.09161.PTDCpt_PT
dc.relation2020/37/B/NZ9/03355pt_PT
dc.relationUID/CVT/00276/2020pt_PT
dc.relationLA/0059/2020/AL4AnimalSpt_PT
dc.relation.publisherversionhttps://www.mdpi.com/2076-2615/13/7/1212pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectEndometrosispt_PT
dc.subjectmarept_PT
dc.subjectcollagenpt_PT
dc.subjectepigeneticspt_PT
dc.subjectDNMTspt_PT
dc.subjectfibroblastspt_PT
dc.subject5-aza-dCpt_PT
dc.subjectdemethylating inhibitorpt_PT
dc.title5-Aza-20 -Deoxycytidine (5-Aza-dC, Decitabine) Inhibits Collagen Type I and III Expression in TGF-β1-Treated Equine Endometrial Fibroblastspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.conferencePlaceBasel, Switzerlandpt_PT
oaire.citation.titleAnimalspt_PT
oaire.citation.volumeVol. 13(7):1212 DOI10.3390/ani1307121pt_PT
person.familyNameLeitão Ferreira-Dias
person.givenNameGraça Maria
person.identifier.ciencia-idF51C-7F45-C720
person.identifier.orcid0000-0003-0622-6513
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication0fb8ec33-7ee1-482b-bdcb-eff773226f90
relation.isAuthorOfPublication.latestForDiscovery0fb8ec33-7ee1-482b-bdcb-eff773226f90

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