A carregar...
Publicação
The use of flow cytometry for fungal nuclear DNA quantification
| Nome: | Descrição: | Tamanho: | Formato: | |
|---|---|---|---|---|
| 1.36 MB | Adobe PDF |
Orientador(es)
Resumo(s)
Genome size information is sparse across fungi, with information being available for less
than 2000 species. So far, most records have been obtained using static, microscopebased
cytometry methods or derived from genome sequencing projects. Flow cytometry
is now considered the state-of-the-art method for obtaining genome size measurements,
and appropriate methods and DNA standards are available, enabling the analysis of most
genome size ranges in a rapid, robust and inexpensive way. The average fungal genome
size is 60 Mbp, but sizes vary across phylogeny, ranging from 2.2 (Encephalitozoon
romaleae) to 3706 Mbp (Jafnea semitosta). In several fungal clades, genome size expansion
seems to accompany evolution either to plant mutualism or to plant parasitism (particularly
biotrophy), and fungi that interact with plants seem to have larger genomes
than saprobes and those that interact with animals. Whereas flow cytometry for nuclear
DNA quantification is routinely employed in plant sciences for genome size and ploidy
studies, its use in fungal biology is still infrequent. Appropriate standards, methods and
best practices are described here, with the aim of stimulating a more generalized and
widespread use of flow cytometry for fungal genome size measurement
Descrição
Palavras-chave
DNA standards flow cytometry genome size mycology nuclear extraction
Contexto Educativo
Citação
Cytometry. 2021;99:343–347
Editora
ISAC