Protocol for the isolation and culture of microglia, astrocytes, and neurons from the same mouse brain

Leites, Elvira; Morais, Vanessa A.

http://hdl.handle.net/10400.5/101153

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Authors

Leites, Elvira

Morais, Vanessa A.

Abstract(s)

Studying the intrinsic properties of microglia, astrocytes, and neurons is essential to our understanding of brain function. Here, we present a protocol to isolate and culture these neural cells from the same mouse brain. Using immunocapture magnetic beads, we describe steps for dissociating, cleaning, and sequentially separating brains from 9-day-old mice into microglia, astrocytes, and neurons. Following these detailed procedures for seeding and culturing of isolated cells, we can address critical questions related to brain function.