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Methicillin resistance of food-borne biofilm-forming staphylococci

2023-07Journal article Open access
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dc.contributor.authorSalamandane, Acácio
dc.contributor.authorCorreia, Jussara
dc.contributor.authorMuetanene, Belo Afonso
dc.contributor.authorSantos, Madalena dos
dc.contributor.authorMalfeito-Ferreira, Manuel
dc.contributor.authorBrito, Luisa
dc.date.accessioned2024-04-03T18:08:06Z
dc.date.available2024-04-03T18:08:06Z
dc.date.issued2023-07
dc.description.abstractThe aim of this study was to evaluate the biofilm-forming ability and the resistance of planktonic cells and biofilm to methicillin (MIC and MBC, and log CFU/cm2 reduction, respectively). Thirty-four isolates were used, including coagulase-positive Staphylococcus (CPS) and coagulasenegative Staphylococcus (CNS), recovered from ready-to-eat (RTE) foods. Biofilm-forming ability was based on enumeration of viable cells from biofilms formed on three types of surfaces, namely stainless steel, polystyrene, and glass. Thirteen of the thirty-four isolates (38.2%) were methicillin-resistant (MIC higher than 4 g/mL). Staphylococcus aureus (CPS) and Staphylococcus saprophyticus (CNS) were the species that showed the highest percentage of resistance (50% and 71.4%, respectively). Staphylococcus warneri was the only species susceptible to methicillin. In 20 of the 34 isolates, the MBC value was twice the MIC value. The CPS (S. aureus) and the CNS (the other species) were not significantly different (p > 0.05) on biofilm-forming ability on the three surfaces used. However, when comparing the averages obtained for the biofilm-forming ability of the isolates, the values obtained (log CFU/cm2) in polystyrene were significantly higher (p < 0.05) than those obtained with the isolates on stainless steel. On the other hand, data from biofilm-forming ability of the isolates on glass and stainless steel are positively and significantly correlated with each other (r = 0.54; p = 0.02). It was not possible to determine the concentration of methicillin that promotes biofilm removal since log reductions were less than 3 log CFU/cm2. The results of this study indicate that foodborne CPS and CNS can form biofilms on different types of material. As these biofilms are resistant to high concentrations of methicillin, their occurrence in food environments and their spread to medical settings can result in staphylococcal food poisoning or, in the worst-case scenario, septicemia, respectively. Good hygiene and good manufacturing practices (GHP and GMP) are therefore mandatory to prevent contamination with Staphylococcus spp.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSalamandane, A.; Correia, J.; Muetanene, B.A.; dos Santos, M.; Malfeito-Ferreira, M.; Brito, L. Methicillin resistance of food-borne biofilm-forming staphylococci. Appl. Sci. 2023, 13, 7725.pt_PT
dc.identifier.doi10.3390/app13137725pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.5/30520
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationIncidência e controlo de bactérias patogénicas em alimentos frescos e de rua em Moçambique
dc.relationLinking Landscape, Environment, Agriculture and Food
dc.relation.publisherversionhttps://www.mdpi.com/journal/applscipt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectfoodborne Staphylococcus spp.pt_PT
dc.subjectbiofilmspt_PT
dc.subjectmethicillin-resistant S. aureus (MRSA)pt_PT
dc.subjectminimum inhibitory concentration—MICpt_PT
dc.subjectminimum bactericidal concentration—MBCpt_PT
dc.titleMethicillin resistance of food-borne biofilm-forming staphylococcipt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleIncidência e controlo de bactérias patogénicas em alimentos frescos e de rua em Moçambique
oaire.awardTitleLinking Landscape, Environment, Agriculture and Food
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//PRT%2FBD%2F151521%2F2021/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04129%2F2020/PT
oaire.citation.issue13pt_PT
oaire.citation.startPageArticle number 7725pt_PT
oaire.citation.titleApplied Sciencespt_PT
oaire.citation.volume13pt_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameSalamandane
person.familyNameMalfeito-Ferreira
person.familyNameBrito
person.givenNameAcácio
person.givenNameManuel
person.givenNameLuisa
person.identifier.ciencia-idB21A-DC37-6D8F
person.identifier.ciencia-id7313-2302-AAB5
person.identifier.ciencia-id2E19-52D0-0BB5
person.identifier.orcid0000-0003-0822-5549
person.identifier.orcid0000-0002-7985-963X
person.identifier.orcid0000-0002-8614-5184
person.identifier.ridB-3215-2019
person.identifier.scopus-author-id6602549195
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublicationec949dd6-d24d-4965-bc9f-6feb421db104
relation.isAuthorOfPublication54fe02c8-ee2a-487e-8c5c-00893cab58c8
relation.isAuthorOfPublication.latestForDiscovery54fe02c8-ee2a-487e-8c5c-00893cab58c8
relation.isProjectOfPublication567b478f-2a93-422a-8470-e6877126a6fc
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