Publication
Local arrangement of Fibronectin by myofibroblasts governs peripheral nuclear positioning in muscle cells
| dc.contributor.author | Roman, William | |
| dc.contributor.author | Martins, João P. | |
| dc.contributor.author | Gomes, Edgar | |
| dc.date.accessioned | 2021-11-29T17:21:10Z | |
| dc.date.available | 2021-11-29T17:21:10Z | |
| dc.date.issued | 2018 | |
| dc.description | © 2018 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/) | pt_PT |
| dc.description.abstract | Skeletal muscle cells (myofibers) are rod-shaped multinucleated cells surrounded by an extracellular matrix (ECM) basal lamina. In contrast to other cell types, nuclei in myofibers are positioned just below the plasma membrane at the cell periphery. Peripheral nuclear positioning occurs during myogenesis and is driven by myofibril crosslinking and contraction. Here we show that peripheral nuclear positioning is triggered by local accumulation of fibronectin secreted by myofibroblasts. We demonstrate that fibronectin via α5-integrin mediates peripheral nuclear positioning dependent on FAK and Src activation. Finally, we show that Cdc42, downstream of restricted fibronectin activation, is required for myofibril crosslinking but not myofibril contraction. Thus we identify that local activation of integrin by fibronectin secreted by myofibroblasts activates peripheral nuclear positioning in skeletal myofibers. | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.citation | Dev Cell. 2018 Jul 2;46(1):102-111.e6 | pt_PT |
| dc.identifier.doi | 10.1016/j.devcel.2018.05.031 | pt_PT |
| dc.identifier.eissn | 1534-5807 | |
| dc.identifier.issn | 1534-5807 | |
| dc.identifier.uri | http://hdl.handle.net/10451/50208 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.publisher | Elsevier | pt_PT |
| dc.relation.publisherversion | https://www.sciencedirect.com/journal/developmental-cell | pt_PT |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | pt_PT |
| dc.subject | Cell-cell interaction | pt_PT |
| dc.subject | Extracellular matrix signaling | pt_PT |
| dc.subject | Fibronectin | pt_PT |
| dc.subject | Muscle development | pt_PT |
| dc.subject | Nuclear movement | pt_PT |
| dc.subject | Nuclear position | pt_PT |
| dc.title | Local arrangement of Fibronectin by myofibroblasts governs peripheral nuclear positioning in muscle cells | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.endPage | 111.e6 | pt_PT |
| oaire.citation.issue | 1 | pt_PT |
| oaire.citation.startPage | 102 | pt_PT |
| oaire.citation.title | Developmental Cell | pt_PT |
| oaire.citation.volume | 46 | pt_PT |
| person.familyName | Roman | |
| person.familyName | Gomes | |
| person.givenName | William | |
| person.givenName | Edgar | |
| person.identifier.ciencia-id | B018-06C2-085E | |
| person.identifier.ciencia-id | 6215-9445-3042 | |
| person.identifier.orcid | 0000-0002-2210-0980 | |
| person.identifier.orcid | 0000-0002-6941-4872 | |
| person.identifier.rid | I-4160-2017 | |
| person.identifier.rid | D-9000-2015 | |
| person.identifier.scopus-author-id | 7102464212 | |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | article | pt_PT |
| relation.isAuthorOfPublication | 68fee748-a304-4464-a76a-8c2e5a1eb9dc | |
| relation.isAuthorOfPublication | bd5022e4-63ff-4d9e-b4f4-3fc163836720 | |
| relation.isAuthorOfPublication.latestForDiscovery | bd5022e4-63ff-4d9e-b4f4-3fc163836720 |
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