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When warming hits harder: survival, cellular stress and thermal limits of Sparus aurata larvae under global change

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dc.contributor.authorMadeira, Diana
dc.contributor.authorCosta, Pedro M.
dc.contributor.authorVinagre, Catarina
dc.contributor.authorDiniz, Mário S.
dc.date.accessioned2020-01-19T18:19:02Z
dc.date.available2020-01-19T18:19:02Z
dc.date.issued2016
dc.description.abstractUnderstanding physiological and molecular compensation mechanisms that shape thermotolerance is crucial for estimating the effects of ocean warming on fish stocks, especially during early life stages, whose tolerance determines recruitment success and population viability. The aims of this study were to assess the sensitivity of fish larvae toward ocean warming and heat wave events in the commercial species, Sparus aurata, whose habitat is likely to be affected by rising water temperatures. We (1) estimated its critical thermal maximum (CTmax) and relative mortality upon warming, (2) quantified stress biomarkers: heat shock protein 70 kDa, total ubiquitin, antioxidant enzymes (superoxide dismutase, catalase, glutathione-S-transferase), lipid peroxidation and protein carbonylation, and (3) analyzed histopathological changes as a result of thermal stress. Larvae showed increasing levels of lethargy with increasing temperature, attaining a cumulative CTmax value of 30 °C. Relative mortality increased upon warming, reaching 80 % at 30 °C. Oxidative damage was higher at moderate temperatures and decreased at 24 °C probably due to a significant increase in superoxide dismutase’s (SODs) activity. Hsp70 chaperone levels also increased at 26 °C, but unfolding persisted at higher temperatures as shown by the increase in total ubiquitin at 26 and 28 °C, indicating protein damage. Skeletal muscle showed disorganization of muscle fibers from 24 °C onwards. Overall, protein denaturation seems to be the major cause of larval mortality, potentially compromising recruitment’s success from 22 °C onwards, since larvae migrate into nursery grounds by spring and summer (i.e., high temperatures), thus hindering the viability of local fish stocks. These data demonstrate that the biochemical homeostasis of fish can be disturbed within an ecologically realistic thermal range and emphasize the risks of rising global temperatures for larval fishes.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1007/s00227-016-2856-4pt_PT
dc.identifier.issn0025-3162
dc.identifier.urihttp://hdl.handle.net/10451/41062
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringerpt_PT
dc.relation.publisherversionhttps://link.springer.com/article/10.1007/s00227-016-2856-4pt_PT
dc.titleWhen warming hits harder: survival, cellular stress and thermal limits of Sparus aurata larvae under global changept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue4pt_PT
oaire.citation.startPage91pt_PT
oaire.citation.titleMarine Biologypt_PT
oaire.citation.volume163pt_PT
person.familyNameMadeira
person.familyNameVinagre
person.familyNameDiniz
person.givenNameDiana
person.givenNameCatarina
person.givenNameMario
person.identifier440666
person.identifier108086
person.identifier.ciencia-id0313-5EB2-1A99
person.identifier.ciencia-id3F13-843A-791B
person.identifier.ciencia-idEA1C-766B-258D
person.identifier.orcid0000-0002-2435-9283
person.identifier.orcid0000-0003-2146-7948
person.identifier.orcid0000-0003-1571-0366
person.identifier.ridN-3228-2013
person.identifier.ridC-7849-2013
person.identifier.scopus-author-id54401593300
person.identifier.scopus-author-id8980557700
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationbd766fe1-ae90-481e-9ce3-dae0f6e7b379
relation.isAuthorOfPublication9adfabdb-a961-4c8f-9bfa-9d3dde0f94da
relation.isAuthorOfPublication180f7459-b65c-4db2-b446-1e84540d99ee
relation.isAuthorOfPublication.latestForDiscovery180f7459-b65c-4db2-b446-1e84540d99ee

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