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Transcriptomic crosstalk between fungal invasive pathogens and their host cells: opportunities and challenges for next-generation sequencing methods

2016Journal article Open access
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dc.contributor.authorEnguita, Francisco J.
dc.contributor.authorCosta, Marina C.
dc.contributor.authorFusco-Almeida, Ana
dc.contributor.authorMendes-Giannini, Maria
dc.contributor.authorLeitão, Ana
dc.date.accessioned2022-03-07T16:41:47Z
dc.date.available2022-03-07T16:41:47Z
dc.date.issued2016
dc.description© 2016 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license ( http://creativecommons.org/licenses/by/4.0/).pt_PT
dc.description.abstractFungal invasive infections are an increasing health problem. The intrinsic complexity of pathogenic fungi and the unmet clinical need for new and more effective treatments requires a detailed knowledge of the infection process. During infection, fungal pathogens are able to trigger a specific transcriptional program in their host cells. The detailed knowledge of this transcriptional program will allow for a better understanding of the infection process and consequently will help in the future design of more efficient therapeutic strategies. Simultaneous transcriptomic studies of pathogen and host by high-throughput sequencing (dual RNA-seq) is an unbiased protocol to understand the intricate regulatory networks underlying the infectious process. This protocol is starting to be applied to the study of the interactions between fungal pathogens and their hosts. To date, our knowledge of the molecular basis of infection for fungal pathogens is still very limited, and the putative role of regulatory players such as non-coding RNAs or epigenetic factors remains elusive. The wider application of high-throughput transcriptomics in the near future will help to understand the fungal mechanisms for colonization and survival, as well as to characterize the molecular responses of the host cell against a fungal infection.pt_PT
dc.description.sponsorshipMarina C. Costa was supported by a postdoctoral fellowship from Fundação para a Ciência e Tecnologia, Portugal (Ref. SFRH/BPD/65131/2009).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationJ Fungi (Basel). 2016 Jan 14;2(1):7pt_PT
dc.identifier.doi10.3390/jof2010007pt_PT
dc.identifier.eissn2309-608X
dc.identifier.urihttp://hdl.handle.net/10451/51637
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationE-SMART: ENGINEERING OF MIRNA TARGET SITES IN CODING TRANSCRIPTS
dc.relation.publisherversionhttps://www.mdpi.com/journal/jofpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectDual RNA-seqpt_PT
dc.subjectFungal pathogenpt_PT
dc.subjectHigh-throughput transcriptomicspt_PT
dc.subjectInvasive infectionpt_PT
dc.subjectNext generation sequencingpt_PT
dc.subjectNon-coding RNAspt_PT
dc.titleTranscriptomic crosstalk between fungal invasive pathogens and their host cells: opportunities and challenges for next-generation sequencing methodspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleE-SMART: ENGINEERING OF MIRNA TARGET SITES IN CODING TRANSCRIPTS
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F65131%2F2009/PT
oaire.citation.issue1pt_PT
oaire.citation.titleJournal of Fungipt_PT
oaire.citation.volume2pt_PT
person.familyNameEnguita
person.familyNameCosta
person.givenNameFrancisco J.
person.givenNameMarina
person.identifier173306
person.identifier.ciencia-idB215-8D49-3218
person.identifier.ciencia-id6818-7810-6CEC
person.identifier.orcid0000-0002-8072-8557
person.identifier.orcid0000-0001-9020-7539
person.identifier.ridA-2347-2009
person.identifier.ridO-1710-2014
person.identifier.scopus-author-id6602119231
person.identifier.scopus-author-id55453276400
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationa101dbd4-6bf5-4546-882e-e2596eddb6b4
relation.isAuthorOfPublicationc4dcab9e-b873-4587-bb30-5203af150404
relation.isAuthorOfPublication.latestForDiscoverya101dbd4-6bf5-4546-882e-e2596eddb6b4
relation.isProjectOfPublication404c868f-1b7d-402a-869d-09c41b4817da
relation.isProjectOfPublication.latestForDiscovery404c868f-1b7d-402a-869d-09c41b4817da

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