Repository logo
 
Publication

Nanoplastics activate a TLR4/p38-mediated pro-inflammatory response in human intestinal and mouse microglia cells

2023-10Journal article Open access
Simple item page
dc.contributor.authorAntunes, Joana
dc.contributor.authorSobral, Paula
dc.contributor.authorMartins, Marta
dc.contributor.authorBranco, Vasco
dc.date.accessioned2024-01-18T15:56:34Z
dc.date.available2024-01-18T15:56:34Z
dc.date.issued2023-10
dc.date.updated2023-10-21T21:51:33Z
dc.description© 2023 The Authors. Published by Elsevier B.V.pt_PT
dc.description.abstractThe crescent presence of nanoplastics in the environment raises concerns regarding their potential impact on health. This study exposed human colon adenocarcinoma cells (HT29) and microglia cells (N9) to nanoplastics (25 nm, 50 nm, and 100 nm Polystyrene) to investigate their inflammatory responses, which are vital for body's defence. Although cytotoxicity remained generally low, HT29 cells exhibited a notable upregulation of p50 and p38 expression, concomitant with elevated TLR4 expression, in contrast with N9 cells that showed a less pronounced upregulation of these proteins. Additionally, nanoplastic exposure increased IL-1ß levels, partially attenuated by pre-exposure to TLR4 or p38 inhibitors. Intriguingly, N9 cells exposed to nanoplastics exhibited substantial increases in iNOS mRNA. This effect was entirely prevented by pre-exposure to TLR4 or p38 inhibitors, while TNF-α mRNA levels remained relatively stable. These findings underscore the potential of nanoplastics to activate inflammatory pathways, with response kinetics varying depending on the cell type.pt_PT
dc.description.sponsorshipThis research was supported by Fundação para a Ciência e Tecnologia, I.P, through: the Ph.D grant (SFRH/BD/144333/2019), JA; Norma Transitória - DL57/2016/CP1376/CT002, VB; through the Scientific Employment Stimulus - Institutional Call (CEECINST/00102/2018), MM; Nanoplastox Project – Nanoplastic Toxicity: from gut inflammation to systemic effects (2022.04884. PTDC); PAHMIX project - Mixtures of Environmental Carcinogens: a molecular approach to improve environmental risk assessment strategies (PTDC/CTA-AMB/29173/2017); MARE - Marine and Environmental Sciences Centre Strategic Project (UIDB/04292/2020), Associate Laboratory ARNET (LA/P/0069/2020) and iMed. ULisboa’s Strategic Project (UID/DTP/04138/2020). The authors acknowledge Isabella Bramatti, Beatriz Matos and Neusa Figueiredo from the Marine and Environmental Sciences Centre and Carolina Carola Alfenim from University of Lisbon for their important assistance. Thanks is due to Katherine Mahoney, for revising the text. The authors acknowledge iMed.ULisboa, Faculty of Pharmacy, University of Lisbon for providing the cells tested in the present study.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationAntunes J, Sobral P, Martins M, Branco V. Nanoplastics activate a TLR4/p38-mediated pro-inflammatory response in human intestinal and mouse microglia cells. Environmental Toxicology and Pharmacology [Internet]. novembro de 2023;104:104298. Disponível em: https://linkinghub.elsevier.com/retrieve/pii/S1382668923002405pt_PT
dc.identifier.doi10.1016/j.etap.2023.104298pt_PT
dc.identifier.slugcv-prod-3371255
dc.identifier.urihttp://hdl.handle.net/10451/61917
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationCEECINST/00102/2018pt_PT
dc.relationMixtures of Environmental Carcinogens: a molecular approach to improve environmental risk assessment strategies.
dc.relationNanoplastic Toxicity: from gut inflammation to systemic effects
dc.relationToxicological Mechanisms of Nanoplastics: Inflammation has a Genotoxicity Trigger
dc.relationMarine and Environmental Sciences Centre
dc.relationAquatic Research Infrastructure Network
dc.relation.publisherversionhttps://linkinghub.elsevier.com/retrieve/pii/S1382668923002405pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectNanoplasticspt_PT
dc.subjectIntestinept_PT
dc.subjectInflammationpt_PT
dc.subjectTLR4pt_PT
dc.subjectMicrogliapt_PT
dc.subjectp38pt_PT
dc.titleNanoplastics activate a TLR4/p38-mediated pro-inflammatory response in human intestinal and mouse microglia cellspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMixtures of Environmental Carcinogens: a molecular approach to improve environmental risk assessment strategies.
oaire.awardTitleNanoplastic Toxicity: from gut inflammation to systemic effects
oaire.awardTitleToxicological Mechanisms of Nanoplastics: Inflammation has a Genotoxicity Trigger
oaire.awardTitleMarine and Environmental Sciences Centre
oaire.awardTitleAquatic Research Infrastructure Network
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FCTA-AMB%2F29173%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/2022.04884.PTDC/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/SFRH%2FBD%2F144333%2F2019/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04292%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0069%2F2020/PT
oaire.citation.startPage104298pt_PT
oaire.citation.titleEnvironmental Toxicology and Pharmacologypt_PT
oaire.citation.volume104pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream3599-PPCDT
oaire.fundingStreamOE
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameVeloso Neves Branco
person.givenNameVasco Rui
person.identifierC-1144-2014
person.identifier.ciencia-id671C-816B-F077
person.identifier.orcid0000-0002-0466-4934
person.identifier.scopus-author-id8271087300
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.cv.cienciaid671C-816B-F077 | Vasco Rui Veloso Neves Branco
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication06d8e0eb-35e5-4d30-90d8-5af2fdab414b
relation.isAuthorOfPublication.latestForDiscovery06d8e0eb-35e5-4d30-90d8-5af2fdab414b
relation.isProjectOfPublication07a017e2-89b1-4f9d-a116-b440f2d73b5a
relation.isProjectOfPublication0a80d75c-ec66-40f8-affe-24170769da6a
relation.isProjectOfPublication77e6d438-0f74-44e4-b81c-9a5251eedbfd
relation.isProjectOfPublication4ef28e92-cf9f-41e8-b87f-2ad3b85428c4
relation.isProjectOfPublicationf9afd86b-f80d-4e66-ba43-9aa12d56c2e3
relation.isProjectOfPublication.latestForDiscovery4ef28e92-cf9f-41e8-b87f-2ad3b85428c4

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Antunes_et_al_2023_Final.pdf
Size:
3.29 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.2 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

FF - CiênciaVitae - Faculdade de Farmácia