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Derepressed sulfate transporters are strongly and rapidly repressed after sulfate addition to sulfur-depleted Vitis cells
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Orientador(es)
Resumo(s)
A cell system of two Vitis species,Vitis vinifera cv. Touriga Nacional and Vitis rupestris, was chosen to study the
response to sulfate deficiency and sulfate resupply through the analysis of sulfate influx and the expression of
sulfate transporter transcripts. Cell suspensions were grown under two sulfate conditions: S sufficient (þS; 1.5
mM of MgSO4) and S deficient ( S). Both species were equally affected by the S-deficient conditions of the
medium. After 24 h in S medium, cells of both species showed a significant increase in sulfate influx rate, which
was maintained throughout the culture cycle, reaching a maximum at days 4–5 in S conditions. The relative
expression of sulfate transporters from V. vinifera and V. rupestris, VvST and VrST, analyzed by real-time PCR,
confirmed a strong derepression of the sulfate transporters in S conditions. The enhanced influx rates and the
upregulation of VvST and VrST were rapidly reversed by the addition of SO2 4 to the S medium. Vitis vinifera
showed a faster response at the protein level measured by the influx rates, while in V. rupestris, the effect was
demonstrated primarily on the relative expression of VrST.
Descrição
Palavras-chave
Vitis derepression gene expression repression sulfate transporter sulfate uptake
Contexto Educativo
Citação
"International Journal of Plant Science". ISSN 1058-5893. 169 (8) 987-997