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Inhibition of Myeloperoxidase Pro-Fibrotic Effect by Noscapine in Equine Endometrium

2023-02-10Artigo científico Acesso aberto
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dc.contributor.authorAmaral, Ana
dc.contributor.authorCebola, Nélio
dc.contributor.authorZóstek-Mioduchowska, Anna
dc.contributor.authorRebordão, Maria Rosa
dc.contributor.authorKordowitzki, Paweł
dc.contributor.authorSkarzynski, Dariusz
dc.contributor.authorFerreira-Dias, Graça
dc.date.accessioned2023-05-21T22:20:24Z
dc.date.available2023-05-21T22:20:24Z
dc.date.issued2023-02-10
dc.descriptionResearch Areas: Biochemistry & Molecular Biology ; Chemistrypt_PT
dc.description.abstractMyeloperoxidase is an enzyme released by neutrophils when neutrophil extracellular traps (NETs) are formed. Besides myeloperoxidase activity against pathogens, it was also linked to many diseases, including inflammatory and fibrotic ones. Endometrosis is a fibrotic disease of the mare endometrium, with a large impact on their fertility, where myeloperoxidase was shown to induce fibrosis. Noscapine is an alkaloid with a low toxicity, that has been studied as an anti-cancer drug, and more recently as an anti-fibrotic molecule. This work aims to evaluate noscapine inhibition of collagen type 1 (COL1) induced by myeloperoxidase in equine endometrial explants from follicular and mid-luteal phases, at 24 and 48 h of treatment. The transcription of collagen type 1 alpha 2 chain (COL1A2), and COL1 protein relative abundance were evaluated by qPCR and Western blot, respectively. The treatment with myeloperoxidase increased COL1A2 mRNA transcription and COL1 protein, whereas noscapine was able to reduce this effect with respect to COL1A2 mRNA transcription, in a time/estrous cycle phase-dependent manner (in explants from the follicular phase, at 24 h of treatment). Our study indicates that noscapine is a promising drug to be considered as an anti-fibrotic molecule to prevent endometrosis development, making noscapine a strong candidate to be applied in future endometrosis therapies.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationAmaral A, Cebola N, Szóstek-Mioduchowska A, Rebordão MR, Kordowitzki P, Skarzynski D, Ferreira-Dias G. 2023. Inhibition of Myeloperoxidase Pro-Fibrotic Effect by Noscapine in Equine Endometrium. International Journal Molecular Science, 24(4):3593. DOI: https://doi.org/ 10.3390/ijms24043593pt_PT
dc.identifier.doi10.3390/ijms24043593pt_PT
dc.identifier.eissn1422-0067
dc.identifier.urihttp://hdl.handle.net/10400.5/27793
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relation.publisherversionhttps://www.mdpi.com/1422-0067/24/4/3593pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectEquinept_PT
dc.subjectendometrosispt_PT
dc.subjectfibrosispt_PT
dc.subjectmyeloperoxidasept_PT
dc.subjectnoscapinept_PT
dc.subjectinhibitionpt_PT
dc.subjectcollagenpt_PT
dc.titleInhibition of Myeloperoxidase Pro-Fibrotic Effect by Noscapine in Equine Endometriumpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.conferencePlaceBasel, Switzerlandpt_PT
oaire.citation.titleInternal Journal of Molecular Sciencespt_PT
oaire.citation.volumeVol. 24(4)pt_PT
person.familyNameAmaral
person.familyNameRebordão Cordeiro Simões Crisóstomo
person.familyNameLeitão Ferreira-Dias
person.givenNameAna
person.givenNameMaria Rosa
person.givenNameGraça Maria
person.identifier.ciencia-idEB18-9420-421B
person.identifier.ciencia-id9515-257A-B35D
person.identifier.ciencia-idF51C-7F45-C720
person.identifier.orcid0000-0002-0075-262X
person.identifier.orcid0000-0002-4389-445X
person.identifier.orcid0000-0003-0622-6513
person.identifier.scopus-author-id56394000500
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication64ab1595-75c6-4b47-b196-ae1d494e6ca1
relation.isAuthorOfPublicationd1fd322a-70b1-4446-afe0-a5a4b9d1f5fa
relation.isAuthorOfPublication0fb8ec33-7ee1-482b-bdcb-eff773226f90
relation.isAuthorOfPublication.latestForDiscoveryd1fd322a-70b1-4446-afe0-a5a4b9d1f5fa

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