Loading...
Publication
Prospeção de variedades de videira na Serra de São Mamede
| Name: | Description: | Size: | Format: | |
|---|---|---|---|---|
| 2.42 MB | Adobe PDF |
Advisor(s)
Abstract(s)
Esta dissertação tem por objetivo a prospeção de amostras representativas da diversidade intravarietal de castas autóctones em vinhas velhas (anteriores a 1980) na Serra de São Mamede e da sua respetiva conservação, com o objetivo de aumentar o espólio genético das castas já existentes na coleção da Associação Portuguesa para a Diversidade da Videira (PORVID) e tentar encontrar variedades que não tenham sido ainda registadas na base de dados Vitis International Variety Catalogue (VIVC).
Foi feita uma prospeção de variedades autóctones em vinhas velhas (entre os 70 e os 150 anos). A primeira fase da presente dissertação passou por encontrar as vinhas através de imagem satélite e de entrevistas a viticultores seniores da região. A segunda fase foi a prospeção e recolha do material genético, que foi elaborada segundo duas abordagens: 1) prospeção acompanhada por um ampelógrafo, onde foram recolhidas amostras de folhas para identificação molecular das plantas-mãe que o ampelógrafo não conseguiu identificar, ou que suscitaram dúvidas na identificação; 2) amostragem aleatória, onde foram recolhidas amostras de folhas de plantas-mãe marcadas para serem analisadas molecularmente com vista à respetiva identificação varietal. A última fase de campo foi elaborada no inverno e consistiu na recolha do material vegetativo para diagnóstico do vírus do enrolamento foliar tipo 3 (GLRaV-3, Grapevine leafroll-associated virus – 3) e para multiplicação e conservação na PORVID.
Foram prospetadas 11 vinhas velhas e no total foram amostradas 241 plantas-mãe, tendo sido identificadas 61 variedades de videira, das quais 19 são variedades potencialmente desconhecidas pois o perfil genético não foi identificado na base de dados Vitis International Variety Catalogue (VIVC). Na prospeção realizada a frequência do vírus do enrolamento foliar tipo 3 foi de 17%.
This dissertation aims to conduct a prospection of representative samples reflecting the intravarietal diversity of autochthones grape varieties found in old vineyards (established prior to 1980) in the serra de São Mamede. The study focuses on their conservation with the purpose of augmenting the genetic repertoire of existing varieties within the collection held by the Portuguese Association for Grapevine Diversity (PORVID). Furthermore, it seeks to identify varieties that have not yet been documented in the Vitis International Variety Catalogue (VIVC). A survey of native varieties in old vineyards (ranging from 70 to 150 years old) was conducted. The first phase of the dissertation involved locating these vineyards using satellite imagery and interviews with senior viticulturists from the region. The second phase focused on prospection and the collection of genetic material, following two approaches: (1) prospection carried out alongside an ampelographer, during which leaf samples were collected for molecular identification of mother plants that the ampelographer could not identify or that raised doubts in the identification process; (2) random sampling, where leaf samples were taken from randomly selected mother plants to be molecularly analyzed for varietal identification. The final field phase took place in winter and involved collecting vegetative material for the diagnosis of grapevine leafroll-associated virus type 3 and for propagation and conservation at PORVID. In total, 11 old vineyards were surveyed, and 241 mother plants were sampled, resulting in the identification of 61 grapevine varieties. Of these, 19 are potentially unknown varieties, as their genetic profiles were not identified in the Vitis International Variety Catalogue (VIVC) database. The incidence of grapevine leafroll-associated virus type 3 in the surveyed vineyards was found to be 17%.
This dissertation aims to conduct a prospection of representative samples reflecting the intravarietal diversity of autochthones grape varieties found in old vineyards (established prior to 1980) in the serra de São Mamede. The study focuses on their conservation with the purpose of augmenting the genetic repertoire of existing varieties within the collection held by the Portuguese Association for Grapevine Diversity (PORVID). Furthermore, it seeks to identify varieties that have not yet been documented in the Vitis International Variety Catalogue (VIVC). A survey of native varieties in old vineyards (ranging from 70 to 150 years old) was conducted. The first phase of the dissertation involved locating these vineyards using satellite imagery and interviews with senior viticulturists from the region. The second phase focused on prospection and the collection of genetic material, following two approaches: (1) prospection carried out alongside an ampelographer, during which leaf samples were collected for molecular identification of mother plants that the ampelographer could not identify or that raised doubts in the identification process; (2) random sampling, where leaf samples were taken from randomly selected mother plants to be molecularly analyzed for varietal identification. The final field phase took place in winter and involved collecting vegetative material for the diagnosis of grapevine leafroll-associated virus type 3 and for propagation and conservation at PORVID. In total, 11 old vineyards were surveyed, and 241 mother plants were sampled, resulting in the identification of 61 grapevine varieties. Of these, 19 are potentially unknown varieties, as their genetic profiles were not identified in the Vitis International Variety Catalogue (VIVC) database. The incidence of grapevine leafroll-associated virus type 3 in the surveyed vineyards was found to be 17%.
Description
Mestrado em Engenharia de Viticultura e Enologia / Instituto Superior de Agronomia, Universidade de Lisboa. Faculdade de Ciências, Universidade do Porto
Keywords
variedades autóctones variabilidade intervarietal variabilidade intravarietal conservação e seleção de genótipos virus foliar do enrolamento do tipo 3 (GLRaV 3) native varieties intervarietal variability intravarietal variability conservation and selection of genotipes Grapevine leafroll associated virus 3 (GLRaV 3)
Pedagogical Context
Citation
Coelho, P.M.L.C.C. Prospeção de variedades de videira na Serra de São Mamede. Lisboa: ISA, 2024, 58 p. Dissertação de Mestrado
Publisher
Instituto Superior de Agronomia, Universidade de Lisboa