Publicação
Topical ocular delivery of nanoparticles with epoetin beta in Wistar Hannover rats
| dc.contributor.author | Silva, Beatriz | |
| dc.contributor.author | Gonçalves, Lídia | |
| dc.contributor.author | Braz, Berta São | |
| dc.contributor.author | Delgado, Esmeralda | |
| dc.date.accessioned | 2023-09-11T15:50:12Z | |
| dc.date.available | 2023-09-11T15:50:12Z | |
| dc.date.issued | 2023-01-27 | |
| dc.date.updated | 2023-02-27T14:09:38Z | |
| dc.description.abstract | Topical instillation of drugs targeting the posterior ocular segment is an expanding area of research. Chitosan and hyaluronic acid have remarkable mucoadhesive properties and potentially enhance pre-corneal retention time after topical instillation. Bearing this in mind, we explored the possibility of delivering epoetin beta (EPOβ) to the posterior segment of the eye in a chitosan-hyaluronic acid (CS/HA-EPOβ) nanoparticulate system using the topical route of administration. Complete ophthalmological examinations, electroretinography and microhematocrit evaluations were performed in Wistar Hannover (WH) rats, before and after topical administration of nanoparticles. The right eye received CS/HA-EPOβ and the left eye received only empty nanocarriers (control). Animals were split into 6 groups and at designated timepoints, all animals from each group (n = 3) were euthanized and both eyes enucleated. Retinal morphology and EPOβ ocular distribution were assessed, respectively, through hematoxylin and eosin (HE) and immunofluorescence staining. After topical administration, no adverse ocular signs were noted and no significant changes either in microhematocrits nor in electroretinographies were detected. During the study, intraocular pressure (IOP) was always kept within physiological range bilaterally. No histological changes were detected in any of the ocular globes. Immunofluorescence enabled the identification of EPOβ in the retina 12 h after the administration, its presence still being detectable at day 21. In conclusion, CS/HA nanoparticles could efficiently deliver EPOβ to the retina of WH rats after topical instillation, being considered biologically safe. Topical administration of this nanoformulation could be a valuable tool for retinal neuroprotection, decreasing risks associated with more invasive routes of administration, being cost effective and also increasing long-term patients’ compliance. | pt_PT |
| dc.description.sponsorship | This research was funded by the Fundação para a Ciência e a Tecnologia (FCT), Portugal (UID/DTP/04138/2019 and UIDB/04138/2020 to iMed.ULisboa, and is has also been funded by national funds through FCT-Fundação para a Ciência e a Tecnologia, I.P., under the Project UIDB/00276/2020 for CIISA and the Project LA/P/0059/2020 for AL4AnimalS); principal investigator grants CEECIND/03143/2017 (L. M. Gonçalves) and Beatriz Silva thanks to FCT for her fellowship SFRH/BD/130476/2017. | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.citation | Silva B, Gonçalves LM, São Braz B, Delgado E. Topical ocular delivery of nanoparticles with epoetin beta in Wistar Hannover rats. Sci Rep [Internet]. 27 de janeiro de 2023;13(1):1559. Disponível em: https://www.nature.com/articles/s41598-023-28845-0 | pt_PT |
| dc.identifier.doi | 10.1038/s41598-023-28845-0 | pt_PT |
| dc.identifier.issn | 2045-2322 | |
| dc.identifier.slug | cv-prod-3147572 | |
| dc.identifier.uri | http://hdl.handle.net/10451/59204 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.publisher | Springer Nature | pt_PT |
| dc.relation | LA/P/0059/2020 | pt_PT |
| dc.relation | Research Institute for Medicines | |
| dc.relation | Research Institute for Medicines | |
| dc.relation | Centre for Interdisciplinary Research in Animal Health | |
| dc.relation | Not Available | |
| dc.relation | Retinal erythropoietin distribution and neuroprotective effect in a nanoparticulate drug delivery system after subconjunctival and topical adminstration in an animal glaucoma model | |
| dc.relation.publisherversion | https://www.nature.com/articles/s41598-023-28845-0 | pt_PT |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt_PT |
| dc.title | Topical ocular delivery of nanoparticles with epoetin beta in Wistar Hannover rats | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.awardNumber | UID/DTP/04138/2019 | |
| oaire.awardNumber | UIDB/04138/2020 | |
| oaire.awardNumber | UIDB/00276/2020 | |
| oaire.awardNumber | CEECIND/03143/2017/CP1476/CT0005 | |
| oaire.awardNumber | SFRH/BD/130476/2017 | |
| oaire.awardTitle | Research Institute for Medicines | |
| oaire.awardTitle | Research Institute for Medicines | |
| oaire.awardTitle | Centre for Interdisciplinary Research in Animal Health | |
| oaire.awardTitle | Not Available | |
| oaire.awardTitle | Retinal erythropoietin distribution and neuroprotective effect in a nanoparticulate drug delivery system after subconjunctival and topical adminstration in an animal glaucoma model | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FDTP%2F04138%2F2019/PT | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04138%2F2020/PT | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00276%2F2020/PT | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/CEEC IND 2017/CEECIND%2F03143%2F2017%2FCP1476%2FCT0005/PT | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F130476%2F2017/PT | |
| oaire.citation.issue | 1 | pt_PT |
| oaire.citation.startPage | 1559 | pt_PT |
| oaire.citation.title | Scientific Reports | pt_PT |
| oaire.citation.volume | 13 | pt_PT |
| oaire.fundingStream | 6817 - DCRRNI ID | |
| oaire.fundingStream | 6817 - DCRRNI ID | |
| oaire.fundingStream | 6817 - DCRRNI ID | |
| oaire.fundingStream | CEEC IND 2017 | |
| person.familyName | Diogo Gonçalves | |
| person.familyName | São Braz | |
| person.familyName | Delgado | |
| person.givenName | Lídia Maria | |
| person.givenName | Berta | |
| person.givenName | Esmeralda | |
| person.identifier | 294532 | |
| person.identifier.ciencia-id | 7211-22BA-86AD | |
| person.identifier.ciencia-id | B11B-C3C7-3ADF | |
| person.identifier.ciencia-id | 8D1E-61D6-0F40 | |
| person.identifier.orcid | 0000-0002-6799-2740 | |
| person.identifier.orcid | 0000-0003-2688-0459 | |
| person.identifier.orcid | 0000-0001-9383-1310 | |
| person.identifier.scopus-author-id | 56008623000 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| rcaap.cv.cienciaid | 7211-22BA-86AD | Lídia Maria Diogo Gonçalves | |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | article | pt_PT |
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