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Analyzing the safety of the parasiticide fungus Mucor circinelloides : first insights on its virulence profile and interactions with the avian gut microbial community

2024-03Artigo científico Acesso aberto
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dc.contributor.authorLozano, João
dc.contributor.authorCunha, Eva
dc.contributor.authorAlmeida, Cristina
dc.contributor.authorNunes, Mónica
dc.contributor.authorDias, Ricardo
dc.contributor.authorVicente, Eduardo
dc.contributor.authorSebastião, Daniela
dc.contributor.authorHenriques, Sérgio
dc.contributor.authorMadeira de Carvalho, Luís
dc.contributor.authorPaz-Silva, Adolfo
dc.contributor.authorOliveira, Manuela
dc.date.accessioned2024-04-17T12:32:34Z
dc.date.available2024-04-17T12:32:34Z
dc.date.issued2024-03
dc.description.abstractParasiticide fungi are considered an accurate, sustainable, and safe solution for the biocontrol of animal gastrointestinal (GI) parasites. This research provides an initial characterization of the virulence of the native parasiticide fungus Mucor circinelloides (FMV-FR1) and an assessment of its impact on birds’ gut microbes. The genome of this fungus was sequenced to identify the genes coding for virulence factors. Also, this fungus was checked for the phenotypic expression of proteinase, lecithinase, DNase, gelatinase, hemolysin, and biofilm production. Finally, an in vivo trial was developed based on feeding M. circinelloides spores to laying hens and peacocks three times a week. Bird feces were collected for 3 months, with total genomic DNA being extracted and subjected to long-read 16S and 25S-28S sequencing. Genes coding for an iron permease (FTR1), iron receptors (FOB1 and FOB2), ADP-ribosylation factors (ARFs) (ARF2 and ARF6), and a GTPase (CDC42) were identified in this M. circinelloides genome. Also, this fungus was positive only for lecithinase activity. The field trial revealed a fecal microbiome dominated by Firmicutes and Proteobacteria in laying hens, and Firmicutes and Bacteroidetes in peacocks, whereas the fecal mycobiome of both bird species was mainly composed of Ascomycetes and Basidiomycetes fungi. Bacterial and fungal alpha-diversities did not differ between sampling time points after M. circinelloides administrations (P = 0.62 and P = 0.15, respectively). Although findings from this research suggest the lack of virulence of this M. circinelloides parasiticide isolate, more complementary in vitro and in vivo research is needed to conclude about the safety of its administration to birds, aiming at controlling their GI parasites.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationLozano J, Cunha E, Almeida C, Nunes M, Dias R, Vicente E, Sebastião D, Henriques S, Madeira de Carvalho L, Paz-Silva A, Oliveira M. 0. Analyzing the safety of the parasiticide fungus Mucor circinelloides: first insights on its virulence profile and interactions with the avian gut microbial community. Microbiol Spectr 0:e04078-23. https://doi.org/10.1128/spectrum.04078-23pt_PT
dc.identifier.doi10.1128/spectrum.04078-23pt_PT
dc.identifier.urihttp://hdl.handle.net/10451/64363
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherAmerican Society for Microbiologypt_PT
dc.relationCIISA/FMV Project UIDB/00276/2020 and LA/P/0059/2020-AL4AnimalS (both funded by FCT), and from the Projects PID2020-120208RB-I00 (MCINN, Spain; FEDER), FEADER 2022/017B (AGACAL, Spain), and ED431B 2021/07 (funded by Consellería de Cultura, Educación e Universidades, Xunta de Galicia, Spain). Additionally, J.L. holds the Ph.D. Research Fellowship 2020.09037.BD (funded by FCT).pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.titleAnalyzing the safety of the parasiticide fungus Mucor circinelloides : first insights on its virulence profile and interactions with the avian gut microbial communitypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.titleMicrobiology Spectrumpt_PT
person.familyNameOliveira
person.givenNameManuela
person.identifier.ciencia-idBE1E-2B3B-92D2
person.identifier.orcid0000-0002-3910-1525
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication0ea8156e-1a61-432a-a7f1-c9009544ebe7
relation.isAuthorOfPublication.latestForDiscovery0ea8156e-1a61-432a-a7f1-c9009544ebe7

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