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Inoculated cell density as a determinant factor of the growth dynamics and metastatic efficiency of a breast cancer murine model

2016Journal article Open access
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dc.contributor.authorGregório, Ana C.
dc.contributor.authorFonseca, Nuno A.
dc.contributor.authorMoura, Vera
dc.contributor.authorLacerda, Manuela
dc.contributor.authorFigueiredo, Paulo
dc.contributor.authorSimões, Sérgio
dc.contributor.authorDias, Sérgio
dc.contributor.authorMoreira, João Nuno
dc.date.accessioned2021-06-23T13:30:14Z
dc.date.available2021-06-23T13:30:14Z
dc.date.issued2016
dc.descriptionCopyright: © 2016 Gregório et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.pt_PT
dc.description.abstract4T1 metastatic breast cancer model have been widely used to study stage IV human breast cancer. However, the frequent inoculation of a large number of cells, gives rise to fast growing tumors, as well as to a surprisingly low metastatic take rate. The present work aimed at establishing the conditions enabling high metastatic take rate of the triple-negative murine 4T1 syngeneic breast cancer model. An 87% 4T1 tumor incidence was observed when as few as 500 cancer cells were implanted. 4T1 cancer cells colonized primarily the lungs with 100% efficiency, and distant lesions were also commonly identified in the mesentery and pancreas. The drastic reduction of the number of inoculated cells resulted in increased tumor doubling times and decreased specific growth rates, following a Gompertzian tumor expansion. The established conditions for the 4T1 mouse model were further validated in a therapeutic study with peguilated liposomal doxorubicin, in clinical used in the setting of metastatic breast cancer. Inoculated cell density was proven to be a key methodological aspect towards the reproducible development of macrometastases in the 4T1 mouse model and a more reliable pre-clinical assessment of antimetastatic therapies.pt_PT
dc.description.sponsorshipAna Cristina Gregório is a student of the international PhD program in Experimental Biology and Biomedicine (PDBEB) from the Institute for Interdisciplinary Research, University of Coimbra and recipient of the fellowship SFRH/BD/51190/2010 from the Portuguese Foundation for Science and Technology (FCT). The work was supported by the grants PTDC/SAU-BMA/121028/2010 (FCT) and UID/NEU/04539/2013 (FEDER/COMPETE 2020/FCT).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationPLoS One. 2016 Nov 7;11(11):e0165817.pt_PT
dc.identifier.doi10.1371/journal.pone.0165817pt_PT
dc.identifier.eissn1932-6203
dc.identifier.urihttp://hdl.handle.net/10451/48721
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherPLOSpt_PT
dc.relationTHERAPEUTIC ACTIVITY OF A TARGETED DRUG DELIVERY APPROACH ON TUMOR METASTASIZATION
dc.relationDesign of novel breast tumor ‘targeted` multifunctional nanoparticles
dc.relationCNC. IBILI
dc.relation.publisherversionhttps://journals.plos.org/plosone/pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.titleInoculated cell density as a determinant factor of the growth dynamics and metastatic efficiency of a breast cancer murine modelpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleTHERAPEUTIC ACTIVITY OF A TARGETED DRUG DELIVERY APPROACH ON TUMOR METASTASIZATION
oaire.awardTitleDesign of novel breast tumor ‘targeted` multifunctional nanoparticles
oaire.awardTitleCNC. IBILI
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F51190%2F2010/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FSAU-BMA%2F121028%2F2010/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FNEU%2F04539%2F2013/PT
oaire.citation.issue11pt_PT
oaire.citation.titlePLOS ONEpt_PT
oaire.citation.volume11pt_PT
oaire.fundingStream5876-PPCDTI
oaire.fundingStream6817 - DCRRNI ID
person.familyNameDias
person.givenNameSérgio
person.identifier.ciencia-idFB10-2BA3-ACEB
person.identifier.orcid0000-0002-7603-4616
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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