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Eimeria vermiformis Infection model of Murine small intestine

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dc.contributor.authorFigueiredo-Campos, Patricia
dc.contributor.authorFerreira, Cristina
dc.contributor.authorBlankenhaus, Birte
dc.contributor.authorVeldhoen, Marc
dc.date.accessioned2021-10-14T11:02:00Z
dc.date.available2021-10-14T11:02:00Z
dc.date.issued2018
dc.descriptionCopyright © 2018 The Authors; exclusive licensee Bio-protocol LLC.pt_PT
dc.description.abstractEimeria vermiformis is a tissue specific, intracellular protozoan that infects the murine small intestinal epithelia, which has been widely used as a coccidian model to study mucosal immunology. This mouse infection model is valuable to investigate the mechanisms of host protection against primary and secondary infection in the small intestine. Here, we describe the generation of an E. vermiformis stock solution, preparation of sporulated E. vermiformis to infect mice and determination of oocysts burden. This protocol should help to establish a highly reproducible natural infection challenge model to study immunity in the small intestine. The information obtained from using this mouse model can reveal fundamental mechanisms of interaction between the pathogen and the immune response, e.g., provided by intraepithelial lymphocytes (IEL) at the basolateral site of epithelial cells but also a variety of other immune cell populations present in the gut.pt_PT
dc.description.sponsorshipThis work was supported by the European Union H2020 ERA project (No. 667824– EXCELLtoINNOV) for work in the Veldhoen laboratory and Fundação para a Ciência e a Tecnologia (FCT) Studentship SFRH/BD/131605/2017 to P.F-C.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationBio Protoc. 2018 Dec 20;8(24):e3122pt_PT
dc.identifier.doi10.21769/BioProtoc.3122pt_PT
dc.identifier.eissn2331-8325
dc.identifier.urihttp://hdl.handle.net/10451/49885
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherBio-protocol LLCpt_PT
dc.relationLeverage research EXCELLence and INNOVation potential of Instituto de Medicina Molecular (IMM) through translational biomedical research in immunity and infection.
dc.relation.publisherversionhttps://bio-protocol.org/pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectEimeria vermiformispt_PT
dc.subjectSmall intestinept_PT
dc.subjectInfection modelpt_PT
dc.subjectCoccidiosispt_PT
dc.subjectApicomplexanpt_PT
dc.subjectMucosal immunologypt_PT
dc.titleEimeria vermiformis Infection model of Murine small intestinept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardNumber667824
oaire.awardNumber68338
oaire.awardTitleLeverage research EXCELLence and INNOVation potential of Instituto de Medicina Molecular (IMM) through translational biomedical research in immunity and infection.
oaire.awardURIinfo:eu-repo/grantAgreement/EC/H2020/667824/EU
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/68338/PT
oaire.citation.issue24pt_PT
oaire.citation.titleBio-Protocolpt_PT
oaire.citation.volume8pt_PT
oaire.fundingStreamH2020
oaire.fundingStreamOE
person.familyNameFigueiredo-Campos
person.familyNameFerreira
person.familyNameBlankenhaus
person.familyNameVeldhoen
person.givenNamePatricia
person.givenNameCristina
person.givenNameBirte
person.givenNameMarc
person.identifierhttps://scholar.google.pt/citations?hl=en&user=7vG1jLIAAAAJ
person.identifier.ciencia-idEC16-8D36-C5F9
person.identifier.ciencia-idFD19-D7A4-E29D
person.identifier.ciencia-id6413-A3A0-C7AD
person.identifier.orcid0000-0002-9808-6230
person.identifier.orcid0000-0001-6746-932X
person.identifier.orcid0000-0003-0987-5228
person.identifier.orcid0000-0002-1478-9562
person.identifier.scopus-author-id8323789000
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameEuropean Commission
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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