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Genomic-based surveillance reveals high ongoing transmission of multi-drug-resistant Mycobacterium tuberculosis in Southern Brazil

2021-10Journal article Restricted access
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dc.contributor.authorSalvato, Richard Steiner
dc.contributor.authorReis, Ana Júlia
dc.contributor.authorSchiefelbein, Sun Hee
dc.contributor.authorGómez, Michael Andrés Abril
dc.contributor.authorSalvato, Stéphanie Steiner
dc.contributor.authorSilva, Larissa Vitória da
dc.contributor.authorCosta, Elis Regina Dalla
dc.contributor.authorUnis, Gisela
dc.contributor.authorDias, Claudia Fontoura
dc.contributor.authorViveiros, Miguel
dc.contributor.authorPortugal, Isabel
dc.contributor.authorGroll, Andrea von
dc.contributor.authorSilva, Pedro Eduardo Almeida da
dc.contributor.authorKritski, Afrânio Lineu
dc.contributor.authorPerdigão, João
dc.contributor.authorRossetti, Maria Lucia Rosa
dc.date.accessioned2023-08-25T19:31:47Z
dc.date.available2023-08-25T19:31:47Z
dc.date.issued2021-10
dc.date.updated2023-03-13T20:25:08Z
dc.description.abstractGenomic-based surveillance on the occurrence of drug resistance and its transmission dynamics has emerged as a powerful tool for the control of tuberculosis (TB). A whole-genome sequencing approach, phenotypic testing and clinical-epidemiological investigation were used to undertake a retrospective population-based study on drug-resistant (DR)-TB in Rio Grande do Sul, the largest state in Southern Brazil. The analysis included 305 resistant Mycobacterium tuberculosis strains sampled statewide from 2011 to 2014, and covered 75.7% of all DR-TB cases identified in this period. Lineage 4 was found to be predominant (99.3%), with high sublineage-level diversity composed mainly of 4.3.4.2 [Latin American and Mediterranean (LAM)/RD174], 4.3.3 (LAM/RD115) and 4.1.2.1 (Haarlem/RD182) sublineages. Genomic diversity was also reflected in resistance of the variants to first-line drugs. A large number of distinct resistance-conferring mutations, including variants that have not been reported previously in any other setting worldwide, and 22 isoniazid-monoresistant strains with mutations described as disputed in the rpoB gene but causing rifampicin resistance generally missed by automated phenotypic tests as BACTEC MGIT. Using a cut-off of five single nucleotide polymorphisms, the estimated recent transmission rate was 55.1%, with 168 strains grouped into 28 genomic clusters. The most worrying fact concerns multi-drug-resistant (MDR) strains, of which 73.4% were clustered. Different resistance profiles and acquisition of novel mutations intraclusters revealed important amplification of resistance in the region. This study described the diversity of M. tuberculosis strains, the basis of drug resistance, and ongoing transmission dynamics across the largest state in Southern Brazil, stressing the urgent need for MDR-TB transmission control state-wide.pt_PT
dc.description.sponsorshipThis study was funded, in part, by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (Grant No. 001) and supported by Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (Grant No. 17/1265–8 INCT-TB). JP is supported by Fundação para a Ciência e Tecnologia through Estímulo Individual ao Emprego Científico (CEECIND/00394/2017).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSalvato RS, Reis AJ, Schiefelbein SH, Gómez MAA, Salvato SS, da Silva LV, et al. Genomic-based surveillance reveals high ongoing transmission of multi-drug-resistant Mycobacterium tuberculosis in Southern Brazil. International Journal of Antimicrobial Agents [Internet]. 1 de outubro de 2021;58(4):106401. Disponível em: https://www.sciencedirect.com/science/article/pii/S0924857921001667pt_PT
dc.identifier.doi10.1016/j.ijantimicag.2021.106401pt_PT
dc.identifier.issn0924-8579
dc.identifier.slugcv-prod-2853881
dc.identifier.urihttp://hdl.handle.net/10451/59031
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationNot Available
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0924857921001667pt_PT
dc.subjectTuberculosispt_PT
dc.subjectDrug resistancept_PT
dc.subjectTransmissionpt_PT
dc.subjectWhole-genome sequencingpt_PT
dc.titleGenomic-based surveillance reveals high ongoing transmission of multi-drug-resistant Mycobacterium tuberculosis in Southern Brazilpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleNot Available
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/CEEC IND 2017/CEECIND%2F00394%2F2017%2FCP1476%2FCT0001/PT
oaire.citation.issue4pt_PT
oaire.citation.startPage106401pt_PT
oaire.citation.titleInternational Journal of Antimicrobial Agentspt_PT
oaire.citation.volume58pt_PT
oaire.fundingStreamCEEC IND 2017
person.familyNameSalvato
person.familyNameViveiros Bettencourt
person.familyNamePortugal
person.familyNameLucas Mota Perdigão
person.givenNameRichard
person.givenNameMiguel
person.givenNameIsabel
person.givenNameJoão Ruben
person.identifiero39byr4AAAAJ
person.identifier.ciencia-idDF17-B4BF-8756
person.identifier.ciencia-idE310-5746-C205
person.identifier.ciencia-id991C-F26A-9843
person.identifier.orcid0000-0002-5701-9437
person.identifier.orcid0000-0001-9676-6251
person.identifier.orcid0000-0002-7843-0006
person.identifier.orcid0000-0002-0339-1305
person.identifier.scopus-author-id57210463758
person.identifier.scopus-author-id7003949212
person.identifier.scopus-author-id24723211800
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.cv.cienciaid991C-F26A-9843 | João Ruben Lucas Mota Perdigão
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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