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Systematic structure-based search for Ochratoxin-degrading enzymes in Proteomes from Filamentous fungi

2021Journal article Open access
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dc.contributor.authorLeitão, Ana Lúcia
dc.contributor.authorEnguita, Francisco J.
dc.date.accessioned2021-10-29T14:12:41Z
dc.date.available2021-10-29T14:12:41Z
dc.date.issued2021
dc.description© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).pt_PT
dc.description.abstractBackground: ochratoxins are mycotoxins produced by filamentous fungi with important implications in the food manufacturing industry due to their toxicity. Decontamination by specific ochratoxin-degrading enzymes has become an interesting alternative for the treatment of contaminated food commodities. Methods: using a structure-based approach based on homology modeling, blind molecular docking of substrates and characterization of low-frequency protein motions, we performed a proteome mining in filamentous fungi to characterize new enzymes with potential ochratoxinase activity. Results: the proteome mining results demonstrated the ubiquitous presence of fungal binuclear zinc-dependent amido-hydrolases with a high degree of structural homology to the already characterized ochratoxinase from Aspergillus niger. Ochratoxinase-like enzymes from ochratoxin-producing fungi showed more favorable substrate-binding pockets to accommodate ochratoxins A and B. Conclusions: filamentous fungi are an interesting and rich source of hydrolases potentially capable of degrading ochratoxins, and could be used for the detoxification of diverse food commodities.pt_PT
dc.description.sponsorshipThis work is supported by the Portuguese Foundation for Science and Technology (FCT) under the framework of the research grant PTDC-MED-GEN-29389-2017.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationBiomolecules. 2021 Jul 16;11(7):1040pt_PT
dc.identifier.doi10.3390/biom11071040pt_PT
dc.identifier.eissn2218-273X
dc.identifier.urihttp://hdl.handle.net/10451/50058
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationPTDC-MED-GEN-29389-2017pt_PT
dc.relation.publisherversionhttps://www.mdpi.com/journal/biomoleculespt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectBiodegradationpt_PT
dc.subjectMycotoxinpt_PT
dc.subjectOchratoxin Apt_PT
dc.subjectOchratoxinasept_PT
dc.subjectStructural biologypt_PT
dc.titleSystematic structure-based search for Ochratoxin-degrading enzymes in Proteomes from Filamentous fungipt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue7pt_PT
oaire.citation.startPage1040pt_PT
oaire.citation.titleBiomoleculespt_PT
oaire.citation.volume11pt_PT
person.familyNameLeitão
person.familyNameEnguita
person.givenNameAna Lúcia
person.givenNameFrancisco J.
person.identifier173306
person.identifier.ciencia-id161E-7E05-5158
person.identifier.ciencia-idB215-8D49-3218
person.identifier.orcid0000-0001-8548-8655
person.identifier.orcid0000-0002-8072-8557
person.identifier.ridA-2347-2009
person.identifier.scopus-author-id7005219737
person.identifier.scopus-author-id6602119231
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication0242c278-36bb-4a70-9c1d-2eee0de185ed
relation.isAuthorOfPublicationa101dbd4-6bf5-4546-882e-e2596eddb6b4
relation.isAuthorOfPublication.latestForDiscovery0242c278-36bb-4a70-9c1d-2eee0de185ed

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