Publicação
Chitosan and Hyaluronic Acid Nanoparticles as Vehicles of Epoetin Beta for Subconjunctival Ocular Delivery
| dc.contributor.author | Silva, Beatriz | |
| dc.contributor.author | Gonçalves, Lídia | |
| dc.contributor.author | Braz, Berta São | |
| dc.contributor.author | Delgado, Esmeralda | |
| dc.date.accessioned | 2023-09-12T18:08:53Z | |
| dc.date.available | 2023-09-12T18:08:53Z | |
| dc.date.issued | 2022-02-18 | |
| dc.date.updated | 2023-02-27T15:27:18Z | |
| dc.description.abstract | Neuroprotection in glaucoma using epoetin beta (EPOβ) has yielded promising results. Our team has developed chitosan-hyaluronic acid nanoparticles (CS/HA) designed to carry EPOβ into the ocular globe, improving the drug’s mucoadhesion and retention time on the ocular surface to increase its bioavailability. In the present in vivo study, we explored the possibility of delivering EPOβ to the eye through subconjunctival administration of chitosan-hyaluronic acid-EPOβ (CS/HA-EPOβ) nanoparticles. Healthy Wistar Hannover rats (n = 21) were split into 7 groups and underwent complete ophthalmological examinations, including electroretinography and microhematocrit evaluations before and after the subconjunctival administrations. CS/HA-EPOβ nanoparticles were administered to the right eye (OD), and the contralateral eye (OS) served as control. At selected timepoints, animals from each group (n = 3) were euthanized, and both eyes were enucleated for histological evaluation (immunofluorescence and HE). No adverse ocular signs, no changes in the microhematocrits (≈45%), and no deviations in the electroretinographies in both photopic and scotopic exams were observed after the administrations (p < 0.05). Intraocular pressure remained in the physiological range during the assays (11–22 mmHg). EPOβ was detected in the retina by immunofluorescence 12 h after the subconjunctival administration and remained detectable until day 21. We concluded that CS/HA nanoparticles could efficiently deliver EPOβ into the retina, and this alternative was considered biologically safe. This nanoformulation could be a promising tool for treating retinopathies, namely optic nerve degeneration associated with glaucoma. | pt_PT |
| dc.description.sponsorship | This research was funded by the Fundação para a Ciência e a Tecnologia (FCT), Portugal (UID/DTP/04138/2019 and UIDB/04138/2020 to iMed.ULisboa, and is has also been funded by national funds through FCT—Fundação para a Ciência e a Tecnologia, I.P., under the Project UIDB/00276/2020); principal investigator grants CEECIND/03143/2017 (L.M. Gonçalves) and Beatriz Silva thanks to FCT for her fellowship SFRH/BD/130476/2017. | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.citation | Silva B, Gonçalves LM, Braz BS, Delgado E. Chitosan and Hyaluronic Acid Nanoparticles as Vehicles of Epoetin Beta for Subconjunctival Ocular Delivery. Marine Drugs [Internet]. 2022 Feb 18;20(2):151. Available from: http://dx.doi.org/10.3390/md20020151 | pt_PT |
| dc.identifier.doi | 10.3390/md20020151 | pt_PT |
| dc.identifier.issn | 1660-3397 | |
| dc.identifier.slug | cv-prod-2779150 | |
| dc.identifier.uri | http://hdl.handle.net/10451/59247 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.publisher | MDPI | pt_PT |
| dc.relation | Research Institute for Medicines | |
| dc.relation | Research Institute for Medicines | |
| dc.relation | Centre for Interdisciplinary Research in Animal Health | |
| dc.relation | Not Available | |
| dc.relation | Retinal erythropoietin distribution and neuroprotective effect in a nanoparticulate drug delivery system after subconjunctival and topical adminstration in an animal glaucoma model | |
| dc.relation.publisherversion | https://www.mdpi.com/1660-3397/20/2/151 | pt_PT |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt_PT |
| dc.subject | nanoparticles | pt_PT |
| dc.subject | mucoadhesion | pt_PT |
| dc.subject | chitosan | pt_PT |
| dc.subject | hyaluronic acid | pt_PT |
| dc.subject | erythropoietin | pt_PT |
| dc.subject | epoetin beta | pt_PT |
| dc.subject | ocular delivery | pt_PT |
| dc.title | Chitosan and Hyaluronic Acid Nanoparticles as Vehicles of Epoetin Beta for Subconjunctival Ocular Delivery | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.awardTitle | Research Institute for Medicines | |
| oaire.awardTitle | Research Institute for Medicines | |
| oaire.awardTitle | Centre for Interdisciplinary Research in Animal Health | |
| oaire.awardTitle | Not Available | |
| oaire.awardTitle | Retinal erythropoietin distribution and neuroprotective effect in a nanoparticulate drug delivery system after subconjunctival and topical adminstration in an animal glaucoma model | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FDTP%2F04138%2F2019/PT | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04138%2F2020/PT | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00276%2F2020/PT | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/CEEC IND 2017/CEECIND%2F03143%2F2017%2FCP1476%2FCT0005/PT | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F130476%2F2017/PT | |
| oaire.citation.issue | 2 | pt_PT |
| oaire.citation.startPage | 151 | pt_PT |
| oaire.citation.title | Marine Drugs | pt_PT |
| oaire.citation.volume | 20 | pt_PT |
| oaire.fundingStream | 6817 - DCRRNI ID | |
| oaire.fundingStream | 6817 - DCRRNI ID | |
| oaire.fundingStream | 6817 - DCRRNI ID | |
| oaire.fundingStream | CEEC IND 2017 | |
| person.familyName | Diogo Gonçalves | |
| person.familyName | São Braz | |
| person.familyName | Delgado | |
| person.givenName | Lídia Maria | |
| person.givenName | Berta | |
| person.givenName | Esmeralda | |
| person.identifier | 294532 | |
| person.identifier.ciencia-id | 7211-22BA-86AD | |
| person.identifier.ciencia-id | B11B-C3C7-3ADF | |
| person.identifier.ciencia-id | 8D1E-61D6-0F40 | |
| person.identifier.orcid | 0000-0002-6799-2740 | |
| person.identifier.orcid | 0000-0003-2688-0459 | |
| person.identifier.orcid | 0000-0001-9383-1310 | |
| person.identifier.scopus-author-id | 56008623000 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| rcaap.cv.cienciaid | 7211-22BA-86AD | Lídia Maria Diogo Gonçalves | |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | article | pt_PT |
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