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Conservation genetics of the Eastern Carpathian endemic Saussurea porcii Degen (Asteraceae)

datacite.subject.fosDepartamento de Biologia Vegetalpt_PT
dc.contributor.advisorSchneeweiss, Gerald M.
dc.contributor.advisorMonteiro, Filipa
dc.contributor.authorÁvila, Catarina de Almeida Diogo de Bettencourt e
dc.date.accessioned2023-12-28T12:04:59Z
dc.date.embargo2025-10-13
dc.date.issued2023
dc.date.submitted2023
dc.descriptionTese de mestrado, Biologia dos Recursos Vegetais, 2023, Universidade de Lisboa, Faculdade de Ciênciaspt_PT
dc.description.abstractA better knowledge of endemic and rare species is crucial for conservation biologists to have information on how and where to prioritize conservation efforts. The reduced genetic diversity in rare and endemic species can have significant consequences for their long-term survival and adaptability to changing environmental conditions. SNPs are key markers for the analysis of genetic variation and the widely adopted RAD-seq offers a cost-effective SNP discovery and does not require prior genomic knowledge. Saussurea porcii, a highly rare and endangered species is endemic to the Eastern Carpathian. Its survival depends on specific wet and calciferous habitat conditions within a specific altitude range. Such conditions are rare, leading to only a few suitable locations for its occurrence. It is also threatened by grazing and climate changes. Nothing is known about its patterns of genetic diversity. In this study, we used RAD-seq to assess for the first time the genetic diversity and population structure of the 5 existing populations for the development of conservation strategies. The populations showed moderate to high differentiation (FST: 0.08 - 0.24). A moderate-to-high genetic diversity was observed (HE (0.15– 0.21) and π (0.17–0.22)). Our study found high within-population and low among-population genetic variation, attributed to outcrossing mating and long life cycles. Negative FIS was observed across all the populations, which can suggest outbreeding depression. HO>HE values in all populations may indicate a recent bottleneck. During LGM glaciations the Eastern Carpathians acted as a refugia, leading to allopatric speciation of S. porcii. The populations Reb and Tro are situated in formerly glaciated areas, while the remaining ones are not. In-situ and ex-situ conservation should be done, but challenges like climate change and grazing may challenge in-situ conservation. Because the results showed a clear genetic separation between all the populations, conservation must be done without mixing individuals from different populations.pt_PT
dc.identifier.tid203485920
dc.identifier.urihttp://hdl.handle.net/10451/61558
dc.language.isoengpt_PT
dc.subjectConservação genéticapt_PT
dc.subjectDiversidade genéticapt_PT
dc.subjectRAD-seqpt_PT
dc.subjectEndemismopt_PT
dc.subjectTeses de mestrado - 2023pt_PT
dc.titleConservation genetics of the Eastern Carpathian endemic Saussurea porcii Degen (Asteraceae)pt_PT
dc.typemaster thesis
dspace.entity.typePublication
rcaap.rightsembargoedAccesspt_PT
rcaap.typemasterThesispt_PT
thesis.degree.nameTese de mestrado em Biologia dos Recursos Vegetaispt_PT

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