Publicação
Antimicrobial activity of bacteriophages towards Pseudomonas aeruginosa isolates obtained from companion animals
| datacite.subject.fos | Departamento de Biologia Vegetal | pt_PT |
| dc.contributor.advisor | Cunha, Eva Sofia Gonçalves da | |
| dc.contributor.advisor | Chambel, Lélia Mariana Marcão, 1964- | |
| dc.contributor.author | Gomes, Margarida Gaspar | |
| dc.date.accessioned | 2024-11-22T10:38:37Z | |
| dc.date.available | 2024-11-22T10:38:37Z | |
| dc.date.issued | 2024 | |
| dc.date.submitted | 2024 | |
| dc.description | Tese de mestrado, Microbiologia Aplicada, 2024, Universidade de Lisboa, Faculdade de Ciências | pt_PT |
| dc.description.abstract | Antimicrobial resistance poses a significant threat to global health, as it reduces the effectiveness of antibiotics and leads to difficult-to-treat infections. Pseudomonas aeruginosa, a common and ubiquitous pathogen, is known for its ability to develop resistance to multiple antibiotics, particularly with the formation of biofilms. Bacteriophage therapy has shown potential for treating bacterial infections in both human and veterinary medicine. This study aimed to evaluate the antimicrobial activity of bacteriophages JG005 and JG024 against multidrug-resistant Pseudomonas aeruginosa isolates obtained from companion animals. The research involved multiple steps, including characterizing the biofilm-forming ability and resistance profile of the P. aeruginosa isolates, determining the host range of the bacteriophages using the bacterial collection and a spot-on-lawn test, evaluating the bacteriophages’ antimicrobial activity against planktonic cultures through a kinetic time-kill assay using various multiplicities of infection (MOI) (1, 10 and 100), and assessing their ability to inhibit and eradicate biofilms formed by the identified biofilmproducing isolates from the bacterial collection. The results revealed that 68.97% (n=20/29) of the isolates were multidrug-resistant and 41.38% (n=12/29) were biofilm producers. Screening results showed that 58.62% (n=17/29) isolates were susceptible to bacteriophage JG005, while 65.52% (n=19/29) were susceptible to JG024. Time-kill assays showed that JG005 inhibited bacterial growth at all MOIs of 94.12% (n=16/17) and JG024 of 94.74% (n=18/19) of the isolates. Regarding biofilm cells, JG005 showed strong antimicrobial suppression for all MOIs in the 4 h treatment in one isolate and JG024 showed strong antimicrobial suppression in one isolate for MOI 100 in the 4 h treatment. This study underscores the potential of bacteriophages as an alternative or adjunctive therapy against antibiotic-resistant P. aeruginosa infections, particularly in the context of planktonic cells, and highlights the need for further research to optimize their use against biofilms. | pt_PT |
| dc.identifier.tid | 203683625 | |
| dc.identifier.uri | http://hdl.handle.net/10400.5/95555 | |
| dc.language.iso | eng | pt_PT |
| dc.subject | Pseudomonas aeruginosa | pt_PT |
| dc.subject | bacteriófago | pt_PT |
| dc.subject | ensaio Time-Kill | pt_PT |
| dc.subject | biofilmes | pt_PT |
| dc.subject | animais de estimação | pt_PT |
| dc.subject | Teses de mestrado - 2024 | pt_PT |
| dc.title | Antimicrobial activity of bacteriophages towards Pseudomonas aeruginosa isolates obtained from companion animals | pt_PT |
| dc.type | master thesis | |
| dspace.entity.type | Publication | |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | masterThesis | pt_PT |
| thesis.degree.name | Tese de mestrado em Microbiologia Aplicada | pt_PT |