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Enzymes present in neutrophil extracellular traps may stimulate the fibrogenic PGF(2 alpha) pathway in the mare endometrium
Publication . Rebordão, Maria Rosa; Amaral, Ana; Fernandes, Carina; Silva, E; Lukasik, Karolina; Szóstek-Mioduchowska, Anna; Bravo, Pedro; Galvão, António; Skarzynski, Dariusz J.; Ferreira-Dias, Graça
Endometrosis is a fibrotic disease in mare endometrium whose pathological
mechanisms remain obscure. Prostaglandin (PG)F2α, despite modulating reproductive physiological
processes, may also provoke local pathological collagen deposition (fibrogenesis). Neutrophil extracellular traps (NETs) released during inflammation have been linked to fibrogenesis in several
tissues. We have previously shown that enzymes found in NETs increase in vitro collagen production
in mare endometrium. In this study, activation of PGF2α-pathway in equine endometrial explants
challenged in vitro by enzymes found in NETs is shown. Our results indicate that both endocrine
microenvironment (estrous cycle phase) and healthy or pathological conditions of endometrial tissues
play an important role in PGF2α-pathway activation. In the endometrium of the follicular phase,
we have observed both high production of PGF2α and/or PGF2α receptor gene transcription under
the action of enzymes found in NETs, both conditions associated with fibrogenesis in other tissues.
Nevertheless, transcription of the PGF2α receptor gene does not appear to be hormone-dependent,
albeit their levels seem to be dependent on endometrial category in the mid-luteal phase. This study
suggests that enzymes existing in NETs may instigate changes on PGF2α mediators, which may
become an additional mechanism of fibrogenesis in mare endometrium.
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Fundação para a Ciência e a Tecnologia
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3599-PPCDT
Número da atribuição
142833