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Projeto de investigação
BREEDing Coffee for AgroForestry Systems
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Publicações
A Transcriptomic Approach to Understanding the Combined Impacts of Supra-Optimal Temperatures and CO2 Revealed Different Responses in the Polyploid Coffea arabica and Its Diploid Progenitor C. canephora
Publication . Marques, Isabel; Fernandes, Isabel; S. Paulo, Octávio; Lidon, Fernando C.; DaMatta, Fábio M.; Ramalho, José C.; Ribeiro-Barros, Ana I.
Understanding the effect of extreme temperatures and elevated air (CO2) is crucial for
mitigating the impacts of the coffee industry. In this work, leaf transcriptomic changes were evaluated
in the diploid C. canephora and its polyploid C. arabica, grown at 25 C and at two supra-optimal
temperatures (37 C, 42 C), under ambient (aCO2) or elevated air CO2 (eCO2). Both species
expressed fewer genes as temperature rose, although a high number of differentially expressed genes
(DEGs) were observed, especially at 42 C. An enrichment analysis revealed that the two species
reacted differently to the high temperatures but with an overall up-regulation of the photosynthetic
machinery until 37 C. Although eCO2 helped to release stress, 42 C had a severe impact on
both species. A total of 667 photosynthetic and biochemical related-DEGs were altered with high
temperatures and eCO2, which may be used as key probe genes in future studies. This was mostly
felt in C. arabica, where genes related to ribulose-bisphosphate carboxylase (RuBisCO) activity,
chlorophyll a-b binding, and the reaction centres of photosystems I and II were down-regulated,
especially under 42 C, regardless of CO2. Transcriptomic changes showed that both species were
strongly affected by the highest temperature, although they can endure higher temperatures (37 C)
than previously assumed.
Understanding the Impact of Drought in Coffea Genotypes: Transcriptomic Analysis Supports a Common High Resilience to Moderate Water Deficit but a Genotype Dependent Sensitivity to Severe Water Deficit
Publication . Fernandes, Isabel; Marques, Isabel; Paulo, Octávio S.; Batista, Dora; Partelli, Fábio L.; Lidon, Fernando C.; DaMatta, Fábio M.; Ramalho, José C.; Ribeiro-Barros, Ana I.
Water scarcity is the most significant factor limiting coffee production, although some
cultivars can still have important drought tolerance. This study analyzed leaf transcriptomes of
two coffee cultivars with contrasting physiological responses, Coffea canephora cv. CL153 and Coffea.
arabica cv. Icatu, subjected to moderate (MWD) or severe water deficits (SWD). We found that
MWD had a low impact compared with SWD, where 10% of all genes in Icatu and 17% in CL153
reacted to drought, being mainly down-regulated upon stress. Drought triggered a genotype-specific
response involving the up-regulation of reticuline oxidase genes in CL153 and heat shock proteins
in Icatu. Responsiveness to drought also included desiccation protectant genes, but primarily,
aspartic proteases, especially in CL153. A total of 83 Transcription Factors were found engaged in
response to drought, mainly up-regulated, especially under SWD. Together with the enrollment
of 49 phosphatases and 272 protein kinases, results suggest the involvement of ABA-signaling
processes in drought acclimation. The integration of these findings with complementing physiological
and biochemical studies reveals that both genotypes are more resilient to moderate drought than
previously thought and suggests the existence of post-transcriptional mechanisms modulating the
response to drought.
A Transcriptomic Approach to Understanding the Combined Impacts of Supra-Optimal Temperatures and CO2 Revealed Different Responses in the Polyploid Coffea arabica and Its Diploid Progenitor C. canephora
Publication . Marques, Isabel; Fernandes, Isabel; Paulo, Octávio S.; Lidon, Fernando C.; DaMatta, Fábio M.; Ramalho, José C.; Ribeiro-Barros, Ana I.
Understanding the effect of extreme temperatures and elevated air (CO2) is crucial for
mitigating the impacts of the coffee industry. In this work, leaf transcriptomic changes were evaluated
in the diploid C. canephora and its polyploid C. arabica, grown at 25 C and at two supra-optimal
temperatures (37 C, 42 C), under ambient (aCO2) or elevated air CO2 (eCO2). Both species
expressed fewer genes as temperature rose, although a high number of differentially expressed genes
(DEGs) were observed, especially at 42 C. An enrichment analysis revealed that the two species
reacted differently to the high temperatures but with an overall up-regulation of the photosynthetic
machinery until 37 C. Although eCO2 helped to release stress, 42 C had a severe impact on
both species. A total of 667 photosynthetic and biochemical related-DEGs were altered with high
temperatures and eCO2, which may be used as key probe genes in future studies. This was mostly
felt in C. arabica, where genes related to ribulose-bisphosphate carboxylase (RuBisCO) activity,
chlorophyll a-b binding, and the reaction centres of photosystems I and II were down-regulated,
especially under 42 C, regardless of CO2. Transcriptomic changes showed that both species were
strongly affected by the highest temperature, although they can endure higher temperatures (37 C)
than previously assumed.
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Entidade financiadora
European Commission
Programa de financiamento
H2020
Número da atribuição
727934