PLASTICGLOBAL - Assessment of plastic-mediated chemicals transfer and effects in food webs of deep, coastal and estuarine ecosystems under global change scenarios

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Ecotoxicological effects of environmental stressors in the mussel Mytilus galloprovincialis

Publication . Gonçalves, Maria João Sintra Coelho; Guilhermino, Lúcia Maria das Candeias; São Braz, Berta Maria Fernandes Ferreira

Mytilus galloprovincialis (Mediterranean mussel) feeds through water filtration and for this reason among others it has been widely used as sentinel species in monitorization studies of environmental contamination and its effects in the marine environment. The objectives of the present study were:(i) to determine water physicochemical parameters and environmental biomarkers in M. galloprovincialis widely used in monitoring studies to assess environmental quality, and the effects of pollution and other environmental stressors in populations of wild organisms; (ii) to assess the toxicity of microplastics (MPs) and deltamethrin (DELT) individually and in mixture to M. galloprovincialis. In order to reach the first objective, mussels were collected in four different sampling sites of the northern Portuguese coastal shore: Vila Praia de Âncora, Carreço, Cabo do Mundo and São Félix da Marinha. In the laboratory, morphometric parameters were determined, and the health status of mussels collected in different sampling sites was compared using the condition index and neurotoxicity, energy production, oxidative stress, lipid oxidative damage and protein oxidative damage biomarkers. The results indicated that mussels collected in certain sampling sites showed a more precarious health status than mussels collected from other sampling sites. To address the second objective, 38 mussels were collected in a sampling site on the shore near the Porto city and transported to the laboratory. Biomarkers were determined in 9 of the mussels. The other organisms were acclimatized to laboratory conditions for 7 days. After this period the biomarkers were determined in 9 specimens. With the other 20 mussels, a short-term toxicity bioassay was performed. The bioassay treatments were: control (artificial seawater); 0.3 mg of MPs; 1 mg/L of DELT; 0.3 mg/L of MPs + 1 mg/L of DELT. After 96h of exposure to test substances, the filtration rate and the previously mentioned biomarkers were determined in all the mussels. The actual concentration of MPs in the water of all treatments containing this test substance was determined in three different times. No significant differences (p > 0.05) among treatments in any biomarker were found. Thus, the concentrations of MPs and DELT tested (individually or in a mixture) did not induce toxic effects on M. galloprovincialis.

2020-06-09Dissertação de mestrado

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Entidade financiadora

Fundação para a Ciência e a Tecnologia

Programa de financiamento

9471 - RIDTI

Número da atribuição

PTDC/MAR-PRO/1851/2014