Epigenetic and metabolic regulation of endothelial heterogeneity

Financiador

Organização

Publicações

Control of endothelial quiescence by FOXO-regulated metabolites

Publication . Andrade, Jorge; Shi, Chenyue; Costa, Ana S. H.; Choi, Jeongwoon; Kim, Jaeryung; Doddaballapur, Anuradha; Sugino, Toshiya; Ong, Yu Ting; Castro, Marco; Zimmermann, Barbara; Kaulich, Manuel; Guenther, Stefan; Wilhelm, Kerstin; Kubota, Yoshiaki; Braun, Thomas; Koh, Gou Young; Grosso, Ana Rita; Frezza, Christian; Potente, Michael

Endothelial cells (ECs) adapt their metabolism to enable the growth of new blood vessels, but little is known how ECs regulate metabolism to adopt a quiescent state. Here, we show that the metabolite S-2-hydroxyglutarate (S-2HG) plays a crucial role in the regulation of endothelial quiescence. We find that S-2HG is produced in ECs after activation of the transcription factor forkhead box O1 (FOXO1), where it limits cell cycle progression, metabolic activity and vascular expansion. FOXO1 stimulates S-2HG production by inhibiting the mitochondrial enzyme 2-oxoglutarate dehydrogenase. This inhibition relies on branched-chain amino acid catabolites such as 3-methyl-2-oxovalerate, which increase in ECs with activated FOXO1. Treatment of ECs with 3-methyl-2-oxovalerate elicits S-2HG production and suppresses proliferation, causing vascular rarefaction in mice. Our findings identify a metabolic programme that promotes the acquisition of a quiescent endothelial state and highlight the role of metabolites as signalling molecules in the endothelium.

2021Artigo científico

Acesso aberto

Ver mais

Entidade financiadora

European Commission

Programa de financiamento

H2020

Número da atribuição

773047